biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 46:467-470, 2003 | DOI: 10.1023/B:BIOP.0000023898.83886.7d

Developmental Histology of Organogenic and Embryogenic Tissue in Picea omorika Culture

S. Budimir1
1 Institute for Biological Research, Belgrade, Serbia

The histological events during adventitious bud and somatic embryo development in explants from Picea omorika seedlings cultivated in vitro have been examined. Meristematic activity in the superficial layers of cotyledons led to bud primordia formation. Bud primordia had characteristic zonal organization with needle primordia arising on the flanks of the meristem. Somatic embryo initial was formed on the cotyledon surface after an unequal transversal division of a cell. The smaller, apical cell gave rise to apical dome and later on secondary, filamentous suspensor while the larger vacuolated cell presented primary suspensor.

Keywords: Serbian spruce; conifers; organogenesis; somatic embryogenesis
Subjects: in vitro culture, somatic embryogenegis; Picea omorica; Serbian spruce, somatic embryogenesis

Published: November 1, 2003  Show citation

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Budimir, S. (2003). Developmental Histology of Organogenic and Embryogenic Tissue in Picea omorika Culture. Biologia plantarum, 46(3), 467-470. doi: 10.1023/B:BIOP.0000023898.83886.7d
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