biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:475-478, 2003 | DOI: 10.1023/A:1024319428619

Effects of Protein Phosphatase Inhibitors and Calcium Antagonists on Self-Incompatible Reaction in Buckwheat

J. Miljuš-Đukić1, S. Ninković1, M. Nešković1
1 Institute for Biological Research "S. Stanković", Belgrade, Yugoslavia

Isolated pistils of dimorphic buckwheat (Fagopyrum esculentum Moench.) flowers were treated with phosphatase inhibitors (ocadaic acid and cantharidin) and with calcium antagonists (verapamil, La3+, and A23187). They were subsequently cross- or self-pollinated, and the growth of pollen tubes was observed under the fluorescence microscope. All treatments suppressed inhibition of pollen tubes growth suggesting that protein phosphatases and calcium signaling may be involved in self-incompatibility signal transduction in buckwheat.

Keywords: Fagopyrum esculentum Moench.; ocadaic acid; cantharidin; verapamil; lanthanum chloride
Subjects: buckwheat, self-incompatibility; calcium antagonists; cantharidin; Fagopyrum esculentum; lanthanum, La3+; ocadaic acid; phosphatase inhibitors; verapamil

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Miljuš-Đukić, J., Ninković, S., & Nešković, M. (2003). Effects of Protein Phosphatase Inhibitors and Calcium Antagonists on Self-Incompatible Reaction in Buckwheat. Biologia plantarum46(3), 475-478. doi: 10.1023/A:1024319428619
Download citation

References

  1. Brewbaker, J.L., Kwack, B.H.: The essential role of calcium ion in pollen germination and pollen tube growth.-Amer. J. Bot. 50: 859-865, 1963. Go to original source...
  2. Dearnaley, J.D.W., Levina, N.N., Lew, R.R., Heath, B.I., Goring, D.R.: Interrelationships between cytoplasmic Ca2+ peaks, pollen hydration and plasma membrane conductance during compatible and incompatible pollinations of Brassica napus papillae.-Plant Cell Physiol. 38: 985-999, 1997. Go to original source...
  3. De Nettancourt, D.: Incompatibility in Angiosperms.-Sex. Plant Reprod. 10: 185-199, 1997. Go to original source...
  4. Dickinson, H.: No stigma attached to male rejection.-Science 286: 1690-1691, 1999. Go to original source...
  5. Franklin-Tong, V.E.: Signaling and the modulation of pollen tube growth.-Plant Cell 11: 727-738, 1999. Go to original source...
  6. Franklin-Tong, V.E., Holdaway-Clarke, T.L., Straatman, R., Kunkel, J.G., Hepler, P.K.: Involvement of extracellular calcium influx in the self-incompatibility response of Papaver rhoeas.-Plant J. 29: 333-345, 2002. Go to original source...
  7. Miljuš-Ðukić, J., Ninković, S., Maksimović, V., Radović, S., Brkljačić, J., Nešković, M.: Effects of protein metabolism inhibitors on SI reaction in buckwheat (Fagopyrum esculentum Moench).-In: Campbell, C., Przybylski, R. (ed.): Proceedings of the VII International Symposium on Buckwheat. Vol. V. Pp. 9-18. International Buckwheat Research Association, Winnipeg 1998.
  8. Obermeyer, G., Klaushofer, H., Nagl, M., Höftberger, M., Bentrup, F.E.: In vitro germination and growth of lily polen tubes is affected by protein phosphatase inhibitors.-Planta 207: 303-312, 1998. Go to original source...
  9. Rundle, S.J., Nasrallah, M.E., Nasrallah, J.E.: Effects of inhibitors of protein serin/threonine phosphatase on pollination in Brassica.-Plant Physiol. 103: 1165-1171, 1993. Go to original source...
  10. Scutt, C.P., Forham-Skelton, A.P., Croy, R.R.D.: Ocadaic acid causes breakdown of self-incompatibility in Brassica olerace: evidence for the involvement of protein phosphateses in the incompatible response.-Sex. Plant Reprod. 6: 282-285, 1993. Go to original source...
  11. Smith, R.D., Walker, J.C.: Plant protein phosphatases.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 101-125, 1996. Go to original source...
  12. Trewavas, A.J., Malho, R.: Ca2+ signalling in plant cell: the big network!-Curr. Opinion Plant Biol. 1: 428-433, 1998. Go to original source...
  13. Wehling, P., Hackauf, B., Wrivke, G.: Phosphorylation of pollen proteins in relation to self-incompatibility in rye (Secale cereale L.).-Sex. Plant Reprod. 7: 67-75, 1994. Go to original source...