Biologia plantarum 46:477-479, 2003 | DOI: 10.1023/B:BIOP.0000023901.49409.7b
Activities of Soluble and Microsomal Farnesyl Diphosphatases in Datura stramonium
- 1 Department of Biotechnology, Agricultural Plant Stress Research Center, Chonnam National University, Gwangju, South Korea
Farnesyl diphosphatase (FDPase; EC 3.1.7.1) produces farnesol from farnesyl diphosphate (FDP) in a reaction that does not require Mg2+. This report shows that FDPase is constitutively expressed at a high level in the soluble and the microsomal fractions of Datura stramonium. Soluble and microsomal FDPase have a similar pH optimum (5.0 - 6.0) and a similar substrate specificity. Geranyl diphosphate (GDP) and geranylgeranyl diphosphate (GGDP) compete for FDP, but isopentenyl diphosphate (IDP), ATP, and para-nitrophenyl phosphate do not. Soluble FDPase activity was highest in fruit and flower followed by root, and leaf.
Keywords: ATP; farnesol; farnesyl diphosphate; geranyl diphosphate; isopentenyl diphosphate; thorn apple
Subjects: Datura stramonium; farnesyl diphosphatase; microsome, farnesyl diphosphatase; thorn apple, farnesyl diphosphatase
Published: November 1, 2003 Show citation
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Ha, S.B., Lee, D.E., Lee, H.J., Song, S.J., & Back, K. (2003). Activities of Soluble and Microsomal Farnesyl Diphosphatases in Datura stramonium. Biologia plantarum, 46(3), 477-479. doi: 10.1023/B:BIOP.0000023901.49409.7b
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