biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:481-485, 2003 | DOI: 10.1023/B:BIOP.0000041050.41513.da

Polar Transport of Indole-3-Acetic Acid in Relation to Rooting in Carnation Cuttings: Influence of Cold Storage Duration and Cultivar

G. Garrido1, M. B. Arnao2, M. Acosta1, J. Sánchez-Bravo1
1 Departamento de Investigación y Selección, Barberet and Blanc, Puerto Lumbreras, Murcia, Spain
2 Departamento de Biología Vegetal (Fisiología Vegetal), Facultad de Biología, Universidad de Murcia, Espinardo, Murcia, Spain

The influence of cold storage of cuttings on the transport and metabolism of indole-3-acetic acid (IAA) and the rooting were studied in two carnation (Dianthus caryophyllus L.) cultivars (Oriana and Elsy), which are known to exhibit very distinct rooting characteristics. The percentage of rooting at 11 d after planting increased with the storage period particularly in Oriana, but the values in Elsy were higher than in Oriana. Auxin transport was measured by applying 3H-IAA to stem sections. Irrespective of the section localization, the oldest node (node) or the basal internode (base), the transport increased as the storage period increased from 2 to 12 weeks in Oriana and from 2 to 8 weeks in Elsy cuttings. The auxin transport rate was higher in bases than in nodes and also in Elsy than in Oriana at a given storage period. IAA oxidation and hydrolyzation of IAA conjugates (determined by extracting the sections with acetonitrile and NaOH once the basipetal IAA movement ceased after a 24 h transport period) showed a negative, highly significant correlation with the amount of IAA transported. Although the rooting percentage and IAA transport were higher in Elsy than in Oriana, the differences in rooting between the cultivars could not be explained solely by differences in IAA transport.

Keywords: auxin metabolism; Dianthus caryophyllus
Subjects: auxins; carnation, rooting of cuttings; cold, polar transport of IAA; cultivar and genotype differences, rooting, IAA; Dianthus caryophyllus; polar transport of IAA; rooting, IAA, cold storage; transport, auxin, cold storage

Published: December 1, 2003  Show citation

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Garrido, G., Arnao, M.B., Acosta, M., & Sánchez-Bravo, J. (2003). Polar Transport of Indole-3-Acetic Acid in Relation to Rooting in Carnation Cuttings: Influence of Cold Storage Duration and Cultivar. Biologia plantarum, 46(4), 481-485. doi: 10.1023/B:BIOP.0000041050.41513.da
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