biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:487-491, 2003 | DOI: 10.1023/B:BIOP.0000041051.81210.9b

The Horse Chestnut Lines Harboring the rol Genes

S. Zdravković-Korać1, D. Ćalić1, P.H. Druart2, Lj. Radojević1
1 Institute for Biological Research, S. Stanković, Belgrade, Serbia and Montenegro, e-mail
2 Department of Biotechnology, CRA, Gembloux, Belgium

An Agrobacterium rhizogenes-mediated transformation system for Aesculus hippocastanum L. has been developed. Wounded androgenic embryos of A. hippocastanum were inoculated with bacteria containing the pRiA4 plasmid, with the uid A sequence as a reporter gene. The hairy roots emerging from the wounded sites of androgenic embryos were isolated and maintained in Murashige and Skoog's (MS) liquid hormone-free medium. Five hairy root lines have been maintained in vitro for 4 years with unchanged growth rate and might be a suitable source for secondary metabolite production. The transformation events have been confirmed by a polymerase chain reaction specific to the rol A, B, C and D genes. The absence of residual contaminating bacteria has been shown by a polymerase chain reaction specific to the vir D1 sequence.

Keywords: Aesculus hippocastanum L.; Agrobacterium rhizogenes; genetic transformation; rol A, B, C and D
Subjects: Aesculus hippocastanum; Agrobacterium-mediated tranformation; Agrobacterium rhizogenes; genetic transformation; hairy root; horse chestnut, harbouring the rol genes; polymerase chain reaction (PCR); root, hairy; transgenic plants

Published: December 1, 2003  Show citation

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Zdravković-Korać, S., Ćalić, D., Druart, P.H., & Radojević, L. (2003). The Horse Chestnut Lines Harboring the rol Genes. Biologia plantarum, 46(4), 487-491. doi: 10.1023/B:BIOP.0000041051.81210.9b
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