biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 46:493-499, 2003 | DOI: 10.1023/B:BIOP.0000041052.31348.a0

Regulation of the Activity of N-1-Naphthylphthalamic Acid Binding Protein by ATP and Phosphatase

Y.-H. Zhang1, T. Xing1, J.F. Hall1, R.D.J. Barker1, M.C. Elliott1
1 The Norman Borlaug Institute for Plant Science Research, De Montfort University, Leicester, United Kingdom

N-1-naphthylphthalamic acid (NPA), an auxin transport inhibitor, was found to bind specifically to a crude membrane preparation from sugar beet seedling leaf cell suspension cultures. The dissociation constant (Kd) and binding protein concentration were found to be 1.71 μmol dm-3 and 220 pmol g-1(membrane protein), respectively. The amount of specific 3H-NPA binding was significantly increased by adding Mg2+ATP to the binding assay solution. Treatment of membrane preparations with acid phosphatase, prior to the NPA binding assay, resulted in lower specific binding. ATP activation and phosphatase inactivation were culture stage dependent. Although a considerable effect could be detected when using cells from day 8 (representing the linear phase), the same treatment did not alter the binding if cells from day 1 (representing lag phase) or day 14 (representing the stationary phase) were used. These observations have strongly highlighted the possible involvement of a phosphorylation and dephosphorylation mechanism in vivo in the regulation of the activity of the NPA binding protein. High phosphatase activity was found in the supernatant, but not in the membrane pellet) after 50 000 g centrifugation. Our present study has indicated that receptor activity could be regulated by a phosphorylation and dephosphorylation mechanism in plants.

Keywords: auxin transport; Beta vulgaris; receptor phosphorylation
Subjects: ATP, regulation of NPA activity; auxin transport inhibitor; auxins; Beta vulgaris; N-1-naphthylphthalamic acid (NPA), auxin transport inhibitor; phosphatases; protein binding NPA; sugar beet, auxin transport inhibitor; transport, auxin inhibitor

Published: December 1, 2003  Show citation

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Zhang, Y.-H., Xing, T., Hall, J.F., Barker, R.D.J., & Elliott, M.C. (2003). Regulation of the Activity of N-1-Naphthylphthalamic Acid Binding Protein by ATP and Phosphatase. Biologia plantarum, 46(4), 493-499. doi: 10.1023/B:BIOP.0000041052.31348.a0
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