biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:501-507, 2003 | DOI: 10.1023/B:BIOP.0000041053.39549.2a

Cloning and Expression Analysis of Salt Responsive Gene from Kandelia candel

W. Huang1,2, X.D. Fang2, G.Y. Li2, Q.F. Lin2, W.M. Zhao1
1 School of Life Sciences and Technology, Xi'an Jiaotong University, Shaanxi, China
2 Institute of Biological Sciences and Technology, Hainan University, Haikou, Hainan, China

Identification of gene expression patterns in mangroves grown under salinity will help to reveal the molecular mechanisms of salt tolerance. Here, 10 cDNAs of genes were isolated from Kandelia candel and identified by representational difference analysis of cDNA (cDNA RDA) under different NaCl concentrations. Of five genes expressed preferentially under salt condition, two were unknown, three were two kinds of low molecular mass heat-shock proteins (sHSPs) and ADP-ribosylation factor, respectively. The expressions of other five genes were repressed under NaCl stress, two encoded cyclophilins, three were tonoplast intrinsic protein, early light-induced protein and 60S ribosomal protein, respectively.

Keywords: mangroves; representational difference analysis of cDNA; salt tolerance
Subjects: heat-shock proteins, analysis of salt responsive gene; Kandelia candel; mangroves, salt tolerance; polymerase chain reaction (PCR); representational difference analysis of cDNA; resistance/tolerance to salinity; salt responsive gene, cloning, expression; salt stress, tolerance; seed, viviparous; thermal shock; viviparous seed

Published: December 1, 2003  Show citation

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Huang, W., Fang, X.D., Li, G.Y., Lin, Q.F., & Zhao, W.M. (2003). Cloning and Expression Analysis of Salt Responsive Gene from Kandelia candel. Biologia plantarum, 46(4), 501-507. doi: 10.1023/B:BIOP.0000041053.39549.2a
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