biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:507-512, 2003 | DOI: 10.1023/A:1024847007935

Spatio-Temporal Variations in Starch Accumulation During Germination and Post-Germinative Growth of Zygotic and Somatic Embryos of Pinus pinaster

M.-N. Jordy1, J.-M. Favre1
1 UMR-INRA IaM, Faculté des Sciences, Université H. Poincaré-Nancy 1, Vandoeuvre, France

During germination and post-germinative growth of Pinus pinaster Ait. seeds, triglycerides are hydrolysed and concurrently the embryo accumulates starch. In this study, the spatio-temporal variation of starch accumulation was described in zygotic embryos associated (ZE+) or not (ZE-) to their megagametophyte and in somatic embryos (SE). In germinating ZE+, starch was accumulated in the growing tissues, following closely the spatio-temporal pattern of triglycerides depletion. In contrast, in ZE- and SE, starch was only found in cortical cells close to the culture medium. In germinating ZE+, the spatio-temporal variations of starch accumulation can be thus interpreted as the result of the changing contact between the megagametophyte and the growing tissues and also of the existing interactions between triglyceride hydrolysis and the allocation of sucrose exported from the megagametophyte.

Keywords: megagametophyte; pine; sugar distribution; triglycerides
Subjects: germination, zygotic and somatic embryos; in vitro culture, somatic embryogenesis; pine, germination and post-germinative growth; Pinus pinaster; starch, accumulation, germination; sugar distribution, germination and post-germinative growth; triglycerides, germination and post-germinative growth; zygotic embryogenesis

Prepublished online: May 1, 2003; Published: December 1, 2003  Show citation

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Jordy, M.-N., & Favre, J.-M. (2003). Spatio-Temporal Variations in Starch Accumulation During Germination and Post-Germinative Growth of Zygotic and Somatic Embryos of Pinus pinaster. Biologia plantarum, 46(4), 507-512. doi: 10.1023/A:1024847007935
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    References

    1. Ching, T.M.: Compositional changes of Douglas fir seeds during germination.-Plant Physiol. 41: 1313-1319, 1966. Go to original source...
    2. Clark, G.: Staining Procedure.-Williams and Wilkins, Baltimore 1981.
    3. Da Silva, P.M.F.R., Eastmond, P.J., Hill, L.M., Smith, A.M., Rawsthorne, S.: Starch metabolism in developing embryos of oil-seed rape.-Planta 203: 480-487, 1997. Go to original source...
    4. Gösslová, M., Svobodová, H., Lipavská, H., Albrechtová, J., Vreugdenhil, D.: Comparing carbohydrate status during seed development and somatic embryo formation.-In Vitro cell. dev. Biol. Plant 37: 24-28, 2001. Go to original source...
    5. King, J.E., Gifford, D.J.: Amino acid utilization in seeds of loblolly pine during germination and early seedling growth: arginine and arginase activity-Plant Physiol. 113: 1125-1135, 1997. Go to original source...
    6. Konrádová, H., Lipavská, H., Albrechtová, J., Vreugdenhil, D.: Sucrose metabolism during somatic and zygotic embryogeneses in Norway spruce: content of soluble saccharides and localisation of key enzyme activities.-J. Plant Physiol. 159: 387-396, 2002. Go to original source...
    7. Jordy, M.N., Danti, S., Favre, J.M., Racchi, M.L.: Histological and biochemical changes in Pinus spp. seeds during germination and post-germinative growth: triacylglycerol distribution and catalase activity.-Aust. J. Plant Physiol. 27: 1109-1117, 2000. Go to original source...
    8. Lipavská, H., Voráčková, Z.: The effect of sugar supply under in vitro conditions on the efficiency of transfer of plants from in vitro to ex vitro conditions.-Biol. Plant. 36: 3-8, 1994.
    9. Margara, J.: La multiplication végétative de la betterave (Beta vulgaris L.) en culture in vitro.-Compt. rend. Acad. Sci. Paris 285: 1041-1044, 1977.
    10. Mohr, H., Schopfer, P.: Plant Physiology.-Springer Verlag, Berlin 1995. Go to original source...
    11. Mullen, R.T., Gifford, D.J.: Isocitrate lyase from germinated loblolly pine megagametophytes: enzyme purification and immunocharacterization.-Plant Physiol. Biochem. 33: 87-95, 1995a.
    12. Mullen, R.T., Gifford, D.J.: Purification and characterization of the glyoxysomal enzyme malate synthase following seed germination in Pinus taeda.-Plant Physiol. Biochem. 33: 639-648, 1995b.
    13. Murphy, J.B., Hammer, M.F.: Starch synthesis and localization in post-germination of Pinus edulis seedlings.-Can. J. Forest. Res. 24: 1457-1463, 1994. Go to original source...
    14. Randolph, L.F.: A new fixing fluid and a revised schedule for the paraffin method in plant cytology.-Stain Technol. 10: 95-96, 1935. Go to original source...
    15. Stone, S.L., Gifford, D.J.: Structural and biochemical changes in loblolly pine (Pinus taeda L.) seeds during germination and early seedling growth. I. Storage protein reserves.-Int. J. Plant Sci. 158: 727-737, 1997. Go to original source...
    16. Stone, S.L., Gifford, D.J.: Structural and biochemical changes in loblolly pine (Pinus taeda L.) seeds during germination and early seedling growth. II. Storage triacylglycerols and carbohydrates.-Int. J. Plant Sci. 160: 663-671, 1999. Go to original source...

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