biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 46:513-517, 2003 | DOI: 10.1023/A:1024899124774

Secondary Somatic Embryogenesis in Abies numidica

B. Vooková1, R. Matú¹ová1, A. Kormu»ák1
1 Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovak Republic

The induction of secondary somatic embryogenesis in Abies numidica De Lann. was achieved. Precotyledonary, cotyledonary, and desiccated cotyledonary embryos were used as explants. Cotyledonary embryos before desiccation were the most suitable. The most beneficial was induction medium Schenk and Hildebrandt (SH) with 1 mg dm-3 thidiazuron and 1000 mg dm-3 myo-inositol. Initiation frequency was from 1 to 34 %. Maturation of somatic embryos was achieved on modified Murashige and Skoog medium supplemented with 40 g dm-3 maltose, 100 g dm-3 polyethylene glycol-4000 and 10 mg dm-3 abscisic acid. Mature somatic embryos after three weeks of desiccation germinated on SH medium with 10 g dm-3 charcoal and 10 g dm-3 sucrose.

Keywords: Algerian fir; auxin; cytokinin; myo-inositol; protein analysis
Subjects: Abies numidica; abscisic acid (ABA); Algerian fir; auxins; cytokinins; in vitro culture, somatic embryogenesis; myo-inositol, somatic embryogenesis; proteins, analysis; thidiazuron

Prepublished online: May 1, 2003; Published: December 1, 2003  Show citation

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Vooková, B., Matú¹ová, R., & Kormu»ák, A. (2003). Secondary Somatic Embryogenesis in Abies numidica. Biologia plantarum, 46(4), 513-517. doi: 10.1023/A:1024899124774
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