biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:529-533, 2003 | DOI: 10.1023/A:1024807426591

Thidiazuron Induced Multiple Shoot Induction and Plant Regeneration from Cotyledonary Explants of Mulberry

T. Dennis Thomas1
1 Post Graduate and Research Department of Botany, St. Thomas College, Kottayam (Dt), Kerala, India

A rapid micropropagation protocol through induced multiple shoots from the cotyledonary explant of mulberry (Morus alba L) is described. The highest number of shoots (20.3) was obtained when explants from 14-d-old embryos were cultured on Murashige and Skoog (MS) medium supplemented with 7 μM thidiazuron for 45 d. Of the three cultivars used, cv. S-36 was the best followed by cv. K-2 and S-1. The shoots were transferred to MS medium supplemented with 5 μM 6-benzylaminopurine for elongation. The elongated shoots were rooted on half strength MS medium containing 1 - 7 μM indole 3-butyric acid or 1-naphthalene acetic acid. The rooted plants were transplanted to soil with 90 % success. The emerged shoot primordia probably initiated from the pre-existing meristems since the shoot bud show definite vascular connection to the major vascular tissue.

Keywords: acclimatization; growth regulators; Morus alba
Subjects: acclimation from in vitro to ex vitro culture; auxins; cotyledonary explants, thidiazuron; in vitro culture, acclimation from in vitro to ex vitro culture; in vitro culture, plant regeneration, organogenesis; Morus alba; mulberry, micropropagation; multiple shoot induction, thidiazuron; thidiazuron

Prepublished online: May 1, 2003; Published: December 1, 2003  Show citation

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Dennis Thomas, T. (2003). Thidiazuron Induced Multiple Shoot Induction and Plant Regeneration from Cotyledonary Explants of Mulberry. Biologia plantarum, 46(4), 529-533. doi: 10.1023/A:1024807426591
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