biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:541-547, 2003 | DOI: 10.1023/B:BIOP.0000041059.10703.11

Variation in Osmoregulation in Differentially Drought-Sensitive Wheat Genotypes Involves Calcium

H. Nayyar1
1 Department of Botany, Panjab University, Chandigarh-, India, E-mail

Two wheat (Triticum aestivum L.) genotypes differing in their sensitivity to water deficit (stress tolerant - C306 and stress susceptible - HD2329) were subjected to osmotic stress for 7 d using polyethylene glycol (PEG-6000; osmotic potential -1.0 MPa), at initial vegetative growth. The plants were either supplemented with 1 mM CaCl2 (Ca2+) alone or along with verapamil (VP; calcium channel blocker) to investigate the involvement of calcium in governing osmoregulation. Relative elongation rate (RER), dry matter (DM) production, water potential (Ψw), electrolyte leakage (EL), contents of proline (Pro) and glycine betaine (GB) and activities of γ-glutamyl kinase (GK) and proline oxidase (PO) in shoots and roots were examined during stress period. C306 showed relatively higher accumulation of Pro while HD2329 accumulated more GB under stress. RER, DM and Ψw were relatively higher in C306 than HD2329. Roots compared to shoots showed lower content of osmolytes but had faster rate of their accumulation. Presence of Ca2+ in the medium increased the activity of GK and decreased that of PO while in the presence of its inhibitor, decrease in activity of both the enzymes was observed. Ca2+ appeared to reduce the damaging effect of stress by elevating the content of Pro and GB, improving the water status and growth of seedlings and minimizing the injury to membranes. The protective effect of Ca2+ was observed to be more in HD2329 than C306.

Keywords: glycine betaine; γ-glutamyl kinase; proline; proline oxidase; Triticum aestivum; verapamil
Subjects: calcium, osmoregulation; cultivar and genotype differences, osmoregulation, drought; γ-glutamyl kinase; glycine betaine; osmoregulation, osmotic stress; osmotic stress, calcium; polyethylene glycol, osmotic stress; proline, osmoregulation, drought; proline oxidase; resistance/tolerance to drought, water stress; Triticum aestivum; verapamil, Ca channel blocker; water potential, osmotic stress; water stress tolerance; wheat, osmoregulation

Published: December 1, 2003  Show citation

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Nayyar, H. (2003). Variation in Osmoregulation in Differentially Drought-Sensitive Wheat Genotypes Involves Calcium. Biologia plantarum, 46(4), 541-547. doi: 10.1023/B:BIOP.0000041059.10703.11
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