biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:543-550, 2003 | DOI: 10.1023/A:1024863511570

Chromosome Banding and DNA Methylation Patterns, Chromatin Organisation and Nuclear DNA Content in Zingeria biebersteiniana

R. Cremonini1, M. Ruffini Castiglione1, V.G. Grif2, V.V. Kotseruba2, E.O. Punina2, A.V. Rodionov2, O.V. Muravenko3, K.V. Popov3, T.E. Samatadze3, A.V. Zelenin3
1 Department of Botanical Sciences, University of Pisa, Pisa, Italy
2 Laboratory of Biosystematics and Cytology, Komarov Botanical Institute, RAS, St. Petersburg, Russia
3 Laboratory of Functional Morphology of Chromosomes, Enghelgardt Institute of Molecular Biology, RAS, Moscow, Russia

Chromosome structure and chromatin organisation of a two-chromosome model cereal Zingeria biebersteiniana (Claus) P. Smirnov were studied: nuclear DNA content was determined by microdensitometric analysis after Feulgen staining; Feulgen absorption at different thresholds of absorbance in interphase nuclei also provided evidence on the organisation of chromatin, allowing quantitative estimation of condensed chromatin within interphasic nucleus. The DNA methylation pattern of Z. biebersteiniana metaphase chromosomes was examined with a specific monoclonal antibody. 5-methyl-cytosine residues are present in several chromosome sites and differences may be present between corresponding regions of homologues. Chromosome banding pattern reveals large bands in the centromeric regions of each chromosome, showing constitutive heterochromatin; by fluorochromes staining pericentromeric blocks are evidenced. After the cold and 9-aminoacridine pre-treatments and after aceto-carmine and aceto-orceine staining, respectively, the metaphase chromosomes were analysed by image analysis system revealing a segmentation of the chromosome body that resembles Giemsa/Reverse banding in animal chromosomes.

Keywords: chromosomes; fluorochromes; image analysis; Poaceae
Subjects: chromatin organisation; chromosome banding; DNA methylation pattern; fluorochromes; image analysis; nucleus, DNA content; Poaceae, chromosome structure, nuclear DNA content; Zingeria biebersteiniana

Prepublished online: May 1, 2003; Published: December 1, 2003  Show citation

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Cremonini, R., Ruffini Castiglione, M., Grif, V.G., Kotseruba, V.V., Punina, E.O., Rodionov, A.V., ... Zelenin, A.V. (2003). Chromosome Banding and DNA Methylation Patterns, Chromatin Organisation and Nuclear DNA Content in Zingeria biebersteiniana. Biologia plantarum, 46(4), 543-550. doi: 10.1023/A:1024863511570
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