biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:549-554, 2003 | DOI: 10.1023/B:BIOP.0000041060.03286.3f

Response of Barley Seedlings to UV-B Radiation as Affected by Proline and NaCl

I.S. Fedina1, K. Georgieva1, I. Grigorova
1 Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria, e-mail

Barley (Hordeum vulgare L. cv. Alfa) seedlings were treated for 4 d before UV-B irradiation with 0.05 mM proline or 150 mM NaCl. UV-B exposure induced synthesis of yellow coloured compounds with maximum absorbance at 438 nm. The content of these compounds was increased in proline-treated and decreased in NaCl-treated plants. UV-B radiation reduced chlorophyll/carotenoids ratio, oxygen evolution rate and photochemical efficiency of PS 2 as estimated by chlorophyll fluorescence and increased proline accumulation, H2O2 generation and lipid peroxidation. Exogenous proline had no effect on the parameters studied and did not change the response of plants to UV-B radiation. NaCl inhibited photochemical efficiency of PS 2, reduced oxygen evolution and increased H2O2 concentration and lipid peroxidation. The combination of NaCl and proline treatment led to lowering the inhibitory effect of NaCl in non UV-B irradiated seedlings. There was not relationship between the level of UV-B-induced compounds and UV-B tolerance of barley seedlings.

Keywords: chlorophyll fluorescence; Hordeum vulgare; hydrogen peroxide; malondialdehyde; oxygen evolution

Published: December 1, 2003  Show citation

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Fedina, I.S., Georgieva, K., & Grigorova, I. (2003). Response of Barley Seedlings to UV-B Radiation as Affected by Proline and NaCl. Biologia plantarum, 46(4), 549-554. doi: 10.1023/B:BIOP.0000041060.03286.3f
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