biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:581-584, 2003 | DOI: 10.1023/B:BIOP.0000041065.85978.6d

Lipid Composition of in vitro Developing Seeds of Brassica campestris L.

N. Sharma1, A. Phutela1, S.P. Malhotra1, R. Singh1
1 Department of Biochemistry, CCS Haryana Agricultural University, Plant Biochemistry and Molecular Biology Laboratory, Hisar-, India

A liquid culture technique has been developed to study lipid metabolism in seeds of Brassica campestris L. grown in vitro from terminal inflorescences detached 4 to 46 days after anthesis. Seeds developed under these conditions exhibited pattern of growth, deposition of storage products and lipid composition similar to those from intact plant.

Keywords: fatty acids; liquid culture; non-polar lipids; polar lipids
Subjects: Brassica campestris; colza, seed development; fatty acids; germination; in vitro culture, from terminal inflorescences; lipid composition, seeds in vitro

Published: December 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Sharma, N., Phutela, A., Malhotra, S.P., & Singh, R. (2003). Lipid Composition of in vitro Developing Seeds of Brassica campestris L. Biologia plantarum46(4), 581-584. doi: 10.1023/B:BIOP.0000041065.85978.6d
Download citation

References

  1. Archana, Sharma, N., Malhotra, S.P., Singh R.: Changes in fatty acid composition in developing seeds of Brassica campestris L.-J. Plant Biol. 26: 165-172, 1999.
  2. Clegg, K.M.: The application of anthrone reagent to the estimation of starch in cereals.-J. Sci. Food Agr. 1: 40-44, 1956. Go to original source...
  3. Collins, F.I., Alexender, D.E., Rodgers, R.C., Silvela, L.: Analysis of oil content of soybean by wide line NMR.-J. amer. Oil Chem. Soc. 44: 708-710, 1967. Go to original source...
  4. Donovan, G.R., Lee, J.W.: The growth of detached wheat heads in liquid culture.-Plant Sci. Lett. 9: 107-113, 1977. Go to original source...
  5. Donovan, G.R., Lee, J.W.: Effect of the nitrogen source on grain development in detached wheat heads in liquid culture.-Aust. J. Plant Physiol. 5: 81-87, 1978. Go to original source...
  6. Eastmond, P.J., Rawsthorne, S.: Coordinate changes in carbon partitioning and plastidial metabolism during the development of oilseed rape embryos.-Plant Physiol. 122: 767-774, 2000. Go to original source...
  7. Hobbs, D.H., Hume, J.H., Polph, C.E., Cooke, D.T.: Changes in lipid composition during floral development of Brassica campestris.- Phytochemistry 42: 335-339, 1996. Go to original source...
  8. Karamdeep, L., Singh, R., Bhullar, S.S.: Accumulation of starch, protein and activities of sucrose cleaving, transaminating enzymes in chickpea pods raised through liquid culture.-Plant Physiol. Biochem. 36: 675-681, 1998. Go to original source...
  9. Murphy, D.J., Cummins, I.: Biosynthesis of seed storage products during embryogenesis in rapeseed, Brassica napus.-Plant Physiol. 135: 63-69, 1989. Go to original source...
  10. Nitsch, J.P.: Growth of excised ovaries in vitro.- Amer. J. Bot. 38: 566-577, 1951. Go to original source...
  11. Norton, G., Harris, J.F.: Compositional changes in developing rapeseed (Brassica napus L.).-Planta 123: 163-174, 1975. Go to original source...
  12. Perry, H.J., Harwood, J.L.: Changes in the lipid content of developing seeds of Brassica napus.- Phytochemistry 32: 1411-1415, 1993. Go to original source...
  13. Rawsthorne, S., Eastmond, P.J., Kang, F., DaSilva, P.M.R., Smith, A.M., Hutchings, D., Emes, M.J.: Carbon partitioning in plastids during development of Brassica napus embryos.-In: Williams, J.P., Khan, M.U., Lem, N.W. (ed.): Physiology, Biochemistry and Molecular Biology of Plant Lipids. Pp. 152-174. Kluwer Academic Publishers, Dordrecht 1996. Go to original source...
  14. Schultz, D.T., Ohlrogge, J.B.: Biosyntheis of triacylglycerol in Thunbergia alata: Additional evidence for involvement of phosphatidyl choline in unusual monoenoic oil production.-Plant Physiol. Biochem. 38: 169-175, 2000. Go to original source...
  15. Singh, R.: Carbon and energy sources for fatty acid biosynthesis in non-photosynthetic plastids of higher plants.-Proc. indian nat. Acad. Sci. B64: 335-354, 1998.
  16. Singh, R., Kaushal, B.: Response of sugar interconversion, starch and protein accumulation to supplied metabolites during pod-filling in chickpea.-Indian J. exp. Biol. 38: 51-55, 2000.
  17. Stobart, A.K., Stymme, S., Shewry, P.R., Napier, I.: Seed lipids.-In: Harwood, T.L. (ed.): Plant Lipid Biosynthesis: Fundamentals and Agricultural Applications. Pp. 223-245. Cambridge University Press, Cambridge 1998.
  18. Stymne, S., Stobart, A.K.: Triacylglycerol biosynthesis.-In: Stumpf, P.K., Conn, E.E. (ed.): The Biochemistry of Plants. Vol. 9. Pp. 175-214. Academic Press, New York 1987. Go to original source...
  19. Topfer. R., Martini, N., Schell.I.: Modification of plant lipid synthesis.-Science 268: 681-686, 1995. Go to original source...
  20. Williams, M., Robertson, E.J., Leech, R.M., Harwood, J.L.: Lipid metabolism in leaves from young wheat (Triticum aestivum cv. Hareward) plants grown at two carbon dioxide levels.-J. exp. Bot. 49: 511-520, 1998. Go to original source...