biologia plantarum

Biologia plantarum 46:589-591, 2003 | DOI: 10.1023/B:BIOP.0000041067.23890.58

Rapid Axillary Bud Proliferation and ex vitro Rooting of Eupatorium triplinerve

K.P. Martin¹

¹ Department of Biotechnology, University of Calicut, Kerala-, India, e-mail

Effective protocol was established for micropropagation of the medicinal plant Eupatorium triplinerve Vahl through rapid axillary bud proliferation and ex vitro rooting. Murashige and Skoog (MS) medium fortified with 8.87 μM benzylaminopurine (BAP) and 2.46 μM indole-3-butyric acid (IBA) was the best for axillary bud proliferation and developed a mean of 8.1 shoots per node. Excision and culture of the node segments of the in vitro shoots on medium supplemented with the same concentration of growth regulators developed more than 30 shoots within 40 d. Shoot multiplication did not exhibit decrease in the number of shoots even at 7^th subculture. Dipping of the basal end of shoots in 2.46 μM IBA solution for 10 d induced roots and its transfer to small pots facilitated the survival of all rooted shoots (100 %). Ex vitro rooting by direct transfer of the shoots from multiplication medium showed 92 % survival.

Keywords: ayapana; benzylaminopurine; clonal propagation; indole-3-butyric acid

Subjects: auxins; axillary bud proliferation; cytokinins; Eupatorium triplinerve; in vitro culture, acclimation from in vitro to ex vitro culture; in vitro culture, micropropagation; rooting ex vitro after in vitro micropropagation

Published: December 1, 2003  Show citation