biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:611-615, 2003 | DOI: 10.1023/A:1024836200183

Differences in Protein Patterns in Suspension Cultures of Taxus cuspidata Induced by Cerium

J.J. Qiao1, Y.J. Yuan1, H. Zhao1, J.C. Wu1
1 Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, P.R. China

The changes in soluble proteins induced by Ce4+ were analyzed in suspension cultures of Taxus cuspidata using two-dimensional polyacrylamide gel electrophoresis. The ultrastructure of cells obviously changed at day 4 after addition of Ce4+. Large amount of nuclear DNA fragments of about 200 bp were observed. Thirteen protein spots were different between the cultures grown with and without Ce4+ at day 4 as well as at day 6 after addition of Ce4+.

Keywords: Ce4+; programmed cell death; protein; two-dimensional gel electrophoresis
Subjects: cerium, protein patterns; in vitro culture, suspension culture; programmed cell death; proteins, patterns, cerium-induced, suspension culture in vitro; Taxus cuspidata

Prepublished online: May 1, 2003; Published: December 1, 2003  Show citation

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Qiao, J.J., Yuan, Y.J., Zhao, H., & Wu, J.C. (2003). Differences in Protein Patterns in Suspension Cultures of Taxus cuspidata Induced by Cerium. Biologia plantarum, 46(4), 611-615. doi: 10.1023/A:1024836200183
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