biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:617-619, 2003 | DOI: 10.1023/A:1024888217021

The Protective Effect of Free Radical Scavengers and Metal Chelators on Polyethylene Glycol-Treated Rice Leaves

S.Y. Hsu1, C.H. Kao2
1 Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China
2 Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

Effect of free radical scavengers and metal chelators on polyethylene glycol (PEG, osmotic potential -1.5 MPa) induced oxidative damage in detached rice leaves was investigated. PEG treatment resulted in a decrease in relative water content and an increase in proline content, and lipid peroxidation. PEG treatment also decreased chlorophyll and protein contents. Free radical scavengers (ascorbate, sodium benzoate, reduced glutathione, and thiourea) retarded and metal chelators [2,2'-bipyridine (BP), 8-hydroxyquinoline, and 1,10-phenanthroline] prevented PEG-induced oxidative damage. Furthermore, the protective effect of BP was reversed by adding Fe2+ and Cu2+, but not by Mn2+ or Zn2+. The protective effect of BP is most likely mediated through chelation of iron. It seems that oxidative damage induced by PEG may require the participation of iron.

Keywords: lipid peroxidation; Oryza sativa; oxidative damage
Subjects: ascorbate, ascorbic acid; 2,2'-bipyridine; chlorophyll, 2,2'-bipyridine; chlorophyll, polyethylene glycol treatment; free radical scavengers; glutathione; 8-hydroxyquinoline; lipid peroxidation; metal chelators; Oryza sativa; oxidative damage, PEG glycol, metal chelators, free radical scavengers; 1,10-phenanthroline; polyethylene glycol; relative water content; rice, polyethylene glycol; sodium benzoate; thiourea

Prepublished online: May 1, 2003; Published: December 1, 2003  Show citation

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Hsu, S.Y., & Kao, C.H. (2003). The Protective Effect of Free Radical Scavengers and Metal Chelators on Polyethylene Glycol-Treated Rice Leaves. Biologia plantarum, 46(4), 617-619. doi: 10.1023/A:1024888217021
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    References

    1. Bates, L.S., Waldren, S.P., Teare, I.D.: Rapid accumulation of free proline for water stress studies.-Plant Soil 39: 205-207, 1973. Go to original source...
    2. Biehler, K., Fock, H.: Evidence for the contribution of Mehler-peroxidase reaction in dissipating excess electrons in drought-stressed wheat.-Plant Physiol. 112: 265-272, 1996. Go to original source...
    3. Boyer, J.S.: Plant productivity and environment.-Science 218: 443-448, 1982. Go to original source...
    4. Bradford, M.M.: A rapid and sensitive method for the quatitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    5. Chien, H.-F., Wang, J.-W., Lin, C.C., Kao, C.H.: Cadmium toxicity of rice leaves is mediated through lipid peroxidation.-Plant Growth Regul. 33: 205-213, 2001. Go to original source...
    6. Dat, J., Van den Abeele, S., Vranova, E., Van Montagu, M., Inze, D., Van Breusegem, F.: Dual action of the active oxygen species during plant stress responses.-Cell. mol. Life Sci. 57: 779-795, 2000. Go to original source...
    7. Fang, W.-C., Kao, C.H.: Inhibition of methyl jasmonate-promoted senescence in rice leaves by a metal chelators, 2,2'-bipyridine.-Plant Growth Regul. 33: 87-93, 2001. Go to original source...
    8. Gutteridge, J.M.C., Rowley, D.A., Halliwell, B.: Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts.-Biochem. J. 199: 263-265, 1981. Go to original source...
    9. Halliwell, B., Gutteridge, J.M.C.: Free Radicals in Biology.-Clarendon Press, Oxford 1988.
    10. Hanson, A.D., Hitz, W.D.: Metabolic responses of mesophytes to plant water deficits.-Annu. Rev. Plant Physiol. 33: 163-203, 1982. Go to original source...
    11. Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichometry of fatty acid peroxidation.-Arch. Biochem. Biophys. 125: 189-198, 1986. Go to original source...
    12. Lin, C.C., Kao, C.H.: Cell wall peroxidase activity, hydrogen peroxide level and NaCl-inhibited root growth of rice seedlings.-Plant Soil 230: 135-143, 2001. Go to original source...
    13. Lin, J.-N., Wang, J.-W., Kao, C.H.: Effect of abscisic acid and water stress on the senescence of detached rice leaves.-Biol. Plant. 42: 313-316, 1999. Go to original source...
    14. Naqui, A., Chance, B.: Reactive oxygen intermediates in biochemistry.-Annu. Rev. Biochem. 55: 127-166, 1986. Go to original source...
    15. Sgherri, C.L.M., Pinzino, C., Navari-Izzo, F.: Sunflower seedlings subjected to increasing stress by water deficit: Changes in O2 production related to composition of thylakoid membranes.-Physiol. Plant. 96: 446-452, 1996. Go to original source...
    16. Smirnoff, N.: The role of active oxygen in the response of plants to water deficit and dessication.-New Phytol. 125: 27-58, 1993. Go to original source...
    17. Strother, S.: The role of free radicals in leaf senescence.-Gerontology 34: 151-156, 1988. Go to original source...
    18. Weatherley, P.E.: Studies in the water relation of cotton plant. I. The field measurement of water deficit in leaves.-New Phytol. 49: 81-97, 1950. Go to original source...
    19. Wintermans, J. F. G. M., De Mots, A.: Spectrophotometric characteristics of chlorophyll a and b and their pheophytins in ethanol.-Biochim. biophys. Acta 109: 448-453, 1965. Go to original source...

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