biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:617-619, 2003 | DOI: 10.1023/B:BIOP.0000041074.81033.3a

Direct Regeneration of Shoots from Hairy Root Cultures of Centaurium erythraea Inoculated with Agrobacterium rhizogenes

A. Subotić1, S. Budimir1, D. Grubišić1, I. Momčilović1
1 Institute for Biological Research "Siniša Stanković", 29. Novembra 142, Belgrade, Serbia and Montenegro, e-mail

Stable transformation and expression of transgenes was achieved in Centaurium erythraea Gillib. using Agrobacterium rhizogenes-mediated system. Five hairy root clones exhibited the transformed phenotype. Shoot regeneration, with green organized structures, was apparent in 4 clones, after the first subculture on Murashige and Skoog (MS) half strength medium. The integration of Ri-plasmid T-DNA was confirmed by polymerase chain reaction (PCR) analyses.

Keywords: adventitious shoot; centaury; Ri-plasmid T-DNA
Subjects: Agrobacterium-mediated tranformation; Agrobacterium rhizogenes; Centaurium erythraea; centaury, hairy root culture; genetic transformation; hairy root; in vitro culture, regeneration, organogenesis; polymerase chain reaction (PCR); root, hairy; transgenic plants

Published: December 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Subotić, A., Budimir, S., Grubišić, D., & Momčilović, I. (2003). Direct Regeneration of Shoots from Hairy Root Cultures of Centaurium erythraea Inoculated with Agrobacterium rhizogenes. Biologia plantarum, 46(4), 617-619. doi: 10.1023/B:BIOP.0000041074.81033.3a
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Jankovid, T., Krstić, D., Savikin-Fodulović, K., Menković, N., GrubiSi6. D.: Comparative investigation of secoiridoid compounds of Centaurium erythraea grown in nature and cultured in vitro.-Pharm. Pharmacol. Lett. 7: 30-32, 1997.
    2. Momcilovid, I., GrubiSid, D., Kjić, M., NeSković, M.: Agrobacterium rhizogenes-mediated transformation and plant regeneration of four Gentiana species.-Plant Cell Tissue Organ Cult. 50:1-6, 1997. Go to original source...
    3. Murashige, T, Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    4. Okasman-Caldentey, K.M., Kivela, O., Hiltmen, R.: Spontaneous shoot organogenesis and plant regeneration from hairy root cultures of Hyoscyanmus muticus.-Plant Sci. 78: 129-136. 1991. Go to original source...
    5. Ramachandra, S., Rao. G., Ravishankar, A.: Plant cell cultures: chemical factories of secondary metabolites.-Biotechnol. Adv. 20:101-153, 2002. Go to original source...
    6. Sul, I.W., Korban, S.S.: A highly efficient method for isolation genomic DNA from plant tissue.-Plant Tissue Cult. Biotechnol. 2: 113-116, 1996.
    7. Tepfer, M., Casse-Dclbart, F.: Agrobacterium rhizogenes as a vector for transforming higher plants.-Microbiol. Sci. 4: 24-28. 1987.

    Return to the content