biologia plantarum

Biologia plantarum 46:633-636, 2003 | DOI: 10.1023/A:1024896418839

Chlorophyllase Activity and Chlorophyll Content in Wild Type and eti 5 Mutant of Arabidopsis thaliana Subjected to Low and High Temperatures

D.T. Todorov¹, E.N. Karanov², A.R. Smith³, M.A. Hall³

¹ Institute of Plant Physiology, Bulgarian Academy of Science, Sofia, Bulgaria

² Institute of Plant Physiology, Bulgarian Academy of Science, Sofia, Bulgaria

³ Institute of Biological Sciences, University of Wales, Ceredigion, UK

Chlorophyll a (Chl a) content and chlorophyllase (Chlase) activity from leaves of wild type (WT) and the ethylene-insensitive mutant (eti 5) of Arabidopsis thaliana (L.) Heynh during temperature stress and plant recovery have been studied. The plants were subjected to temperatures of 4 °C (LT) and 38 °C (HT) for 24 h. Chl a gradually decreased somewhat during stress and in the first day of recovery, especially in HT-treated plants. At the end of the experimental period (1 d stress and 10 d recovery) Chl a content was lower in eti 5 plants than in WT ones. The Chlase in WT was more affected than in eti 5 plants during the temperature treatment and the recovery period.

Keywords: chilling stress; ethylene-insensitive mutant; heat stress

Subjects: Arabidopsis thaliana; chilling stress; chlorophyll, low and high temperatures; chlorophyllase; heat stress, Arabidopsis; mutant, ethylene-insensitive, Arabidopsis

Prepublished online: May 1, 2003; Published: December 1, 2003  Show citation