biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 48:1-5, 2004 | DOI: 10.1023/B:BIOP.0000024267.68394.96

Changes in Peroxidases and Catalase Activity During in vitro Rooting

A.N. Molassiotis1,*, K. Dimassi1, G. Diamantidis2, I. Therios1
1 Laboratory of Pomology, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece
2 Laboratory of Agricultural Chemistry, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece

Enzyme changes in non-rooted (treated with Fe-EDTA) and rooted (treated with Fe-EDDHA) stems of rootstock GF-677 (Prunus amygdalus×P. persica) during adventitious root formation in vitro have been recorded. The first roots appeared approximately after 12 d on the rooting medium. By contrast to non-rooted stems, rooted stems showed a maximum of soluble peroxidase activity on the 9th day, of ionically bound peroxidase to cell wall on the 6th and 12th day and of catalase on the 6th and the 15th day. A time course study of changes of soluble peroxidases isoenzymes showed that there was a band visible only in the rooted stems and also a new band appeared three days before the emergence of roots.

Keywords: GF-677 (Prunus amygdalus×P. persica); iron
Subjects: catalase; Fe-ethylenediamine-di-(o-hydroxyphenyl)acetic acid; Fe-ethylenediaminetetraacetic acid; in vitro culture, nutrient media modification; in vitro culture, root, rooting; iron, rooting in vitro; nutrient medium, Murashige and Skoog; peroxidase; Prunus amygdalus × Prunus persica; rootstock explants, iron, in vitro culture; rootstock, Prunus amygdalus × Prunus persica

Published: March 1, 2004  Show citation

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Molassiotis, A.N., Dimassi, K., Diamantidis, G., & Therios, I. (2004). Changes in Peroxidases and Catalase Activity During in vitro Rooting. Biologia plantarum, 48(1), 1-5. doi: 10.1023/B:BIOP.0000024267.68394.96
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