biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:7-11, 2004 | DOI: 10.1023/B:BIOP.0000024268.20248.33

Culture Tube Closure-Type Affects Potato Plantlets Growth and Chlorophyll Contents

A. Chanemougasoundharam1, D. Sarkar1, S.K. Pandey1,*, F. Al-Biski3, O. Helali3, J.S. Minhas2
1 Division of Crop Improvement, Central Potato Research Institute, Shimla, Himachal Pradesh, India
2 Division of Crop Physiology and Post-Harvest Technology, Central Potato Research Institute, Shimla, Himachal Pradesh, India
3 GOSM, General Organization of Seed Multiplication (, Aleppo, Syria

The effect of different hermetic and non-hermetic closure-types (aluminum foil, cotton bung, cotton plug, polypropylene cap and Steristopper) on potato (Solanum tuberosum L.) plantlets growth and chlorophyll contents was studied in three genotypes belonging to different maturity groups. Plantlets grown in culture tubes closed with aluminum foils and polypropylene caps had higher fresh mass and shoot length, but lower chlorophyll contents, higher senescence index and various morphological abnormalities. Non-hermetic closures like cotton plugs and Steristoppers were found optimum for plant growth without any morphological abnormalities. Besides, these plantlets exhibited low senescence index and had higher chlorophyll contents that favour acclimation to ex vitro conditions.

Keywords: gas exchange; micropropagation; Solanum tuberosum; tissue culture; vessel closure
Subjects: chlorophyll, in vitro culture, cultivar differences, vessel closure; cultivar and genotype differences, drought, heat shock protein; gas exchange, in vitro culture; in vitro culture, vessel closure-types; nutrient medium, Murashige and Skoog; potato, in vitro culture; Solanum tuberosum; vessel closure types, in vitro culture

Published: March 1, 2004  Show citation

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Chanemougasoundharam, A., Sarkar, D., Pandey, S.K., Al-Biski, F., Helali, O., & Minhas, J.S. (2004). Culture Tube Closure-Type Affects Potato Plantlets Growth and Chlorophyll Contents. Biologia plantarum, 48(1), 7-11. doi: 10.1023/B:BIOP.0000024268.20248.33
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    References

    1. Barnes, J.D., Balaguer, L., Manrique, E., Elvira, S., Davison, A.W.: A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a, and b in lichens and higher plants.-Environ. exp. Bot. 32: 85-100, 1992. Go to original source...
    2. Bottcher, I., Zoglauer, K., Goring, H.: Induction and reversion of vitrification of plants cultured in vitro.-Physiol. Plant. 72: 560-564, 1988.
    3. Debergh, P., Harbaoui, Y., Lemeu, R.R.: Mass propagation of globe artichoke (Cynara scolymus). Evaluation of different hypotheses to overcome vitrification with special reference to water potential.-Physiol. Plant. 53: 181-187, 1981. Go to original source...
    4. During, H, Harst, M.: Stomatal behaviour, photosynthesis and photorespiration of in vitro-grown grapevines: effects of light and CO2.-Vitis 35: 163-167, 1996.
    5. Genoud-Gourichon, C., Veisseire, P., Coudret, A.: Effects of pre-treatment and cap closure on photosynthesis potentialities of potato cultivated in vitro.-Photosynthetica 29: 73-79, 1993.
    6. Haisel, D., Pospíšilová, J., Synková, H., Čatský, J., Wilhelmová, N., Plzáková, š.: Photosynthetic pigments and gas exchange of in vitro grown tobacco plants as affected by CO2 supply.-Biol. Plant. 42: 463-468, 1999. Go to original source...
    7. Hussey, G., Stacey, N. J.: Factors affecting the formation of in vitro tubers in potato (Solanum tuberosum L.).-Ann. Bot. 53: 565-578, 1984. Go to original source...
    8. Jackson, M.B., Abbott, A.J., Belcher, A.R., Hall, K.C., Butler, R., Cameron, J.: Ventilation in plant tissue culture and effects of poor aeration on ethylene and carbon dioxide accumulation, oxygen depletion and explant development.-Ann. Bot. 67: 229-237, 1991. Go to original source...
    9. Kozai, T.: Photoautotrophic micropropagation.-In Vitro cell. dev. Biol. Plant. 27: 47-51, 1991. Go to original source...
    10. Kubota, C., Kozai, T.: Growth and net photosynthetic rate of Solanum tuberosum in vitro under forced and natural ventilation.-HortScience 27: 1312-1314, 1991. Go to original source...
    11. Lentini, Z., Mussell, H., Mutschler, M.A., Earle, E.D.: Ethylene generation and reversal of ethylene effects during development in vitro of rapid-cycling Brassica campestris L.-Plant Sci. 54: 75-81, 1988. Go to original source...
    12. Lucchesini, M., Sodi, A. M.: Effects of vessel permeability to gas exchanges on the in vitro plantlets of Myrtus communis L.-Agr. mediter. 130: 78-84, 2000.
    13. McClelland, M.T., Smith, M.A.L.: Vessel type, closure, and explant orientation influence in vitro performance of five woody species.-HortScience 25: 797-800, 1990. Go to original source...
    14. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    15. Perl, A.D., Aviv, D., Galun, E.: Ethylene and in vitro culture of potato: Suppression of ethylene generation vastly improves protoplast yield, plating efficiency and transient expression of an alien gene.-Plant Cell Rep. 7: 403-406, 1988. Go to original source...
    16. Pospíšilová, J., Haisel, D., Synková, H., Čatský, J., Wilhelmová, N., Plzáková, š., Procházková, D., šrámek, F.: Photosynthetic pigments and gas exchange during ex vitro acclimation of tobacco plants as affected by CO2 supply and abscisic acid.-Plant Cell Tissue Organ Cult. 61: 125-133, 2000. Go to original source...
    17. Pospíšilová, J., Solárová, J., Čatský, J.: Photosynthetic responses to stresses during in vitro cultivation.-Photosynthetica 26: 3-18, 1992.
    18. Sarkar, D., Naik, P.S., Chandra, R.: Effect of inoculation density on potato micropropagation.-Plant Cell Tissue Organ Cult. 48: 63-66, 1997. Go to original source...
    19. Sarkar, D., Kaushik, S.K., Naik, P.S.: Minimal growth conservation of potato microplants: silver thiosulfate reduces ethylene-induced growth abnormalities during prolonged storage in vitro.-Plant Cell Rep. 18: 897-903, 1999. Go to original source...
    20. Sarkar, D., Sood, K.C., Chakrabarti, S.K., Naik, P.S.: Growing of potato microplants in the presence of alginate silverthiosulfate capsules reduces ethylene-induced culture abnormalities during minimal growth conservation in vitro.-Plant Cell Tissue Organ Cult. 68: 79-89, 2002. Go to original source...
    21. Serret, M.D., Trillas, M.I., Matas, J., Aruas, J.L.: The effect of different closure types, light, and sucrose concentrations on carbon isotope composition and growth of Gardenia jasminoides plantlets during micropropagation and subsequent acclimation ex vitro.-Plant Cell Tissue Organ. Cult. 47: 217-230, 1997. Go to original source...
    22. Smith, E.F., Roberts, A.V., Mottley, J.: The preparation in vitro of chrysanthemum for transplantation to soil. 3. Improved resistance to desiccation conferred by reduced humidity.-Plant Cell Tissue Organ Cult. 21: 141-145, 1990. Go to original source...
    23. Solárová, J.: Photosynthesis of plant regenerants. Diurnal variation in CO2 concentration in cultivation vessels resulting from plantlets photosynthetic activity.-Photosynthetica 23: 100-107, 1989.
    24. Steel, R.G.D., Torrie, J.H.: Principles and Procedures of Statistics: A Biometrical Approach.-McGraw Hill, New York 1980.
    25. Zobayed, S.M.A., Amstrong, J., Amstrong, W.: Micropropagation of potato: Evaluation of closed, diffusive and forced ventilation on growth and tuberization.-Ann. Bot. 87: 53-59, 2001. Go to original source...

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