biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:19-23, 2004 | DOI: 10.1023/B:BIOP.0000024270.02186.1f

Genotype- and Sex-Specific Protocols for in vitro Micropropagation and Medium-Term Conservation of Jojoba

R.K. Tyagi1,*, S. Prakash2
1 Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, New Delhi, India
2 Institute for Applied Research, Ben-Gurion University of the Negev, Beer-Sheeva, Israel

Nodal explant cultures from field-grown five jojoba genotypes (EC 99690, EC 99692, EC 99692, EC 267779 and EC 171284; male and female plants), could be established on Murashige and Skoog (MS) medium. The nodal explants of different genotypes as well as sex elicited differential requirements of N6-benzyladenine (BA) for optimum shoot regeneration and medium-term conservation. Female nodal explants of EC 99692 produced maximum shoots (10 shoots per explant) followed by male of EC 171284 (9.3 shoots per explant) on MS + 10 μM BA. The pulse treatment of 50 μM indole-3-butyric acid for 20 min caused in vitro rhizogenesis in 44 - 67 % cultures of various genotypes tested. A significant difference was observed for the conservation period of male and female cultures of all the genotypes. MS + 10 μM BA supported the shoot cultures of EC 99690, EC 99691 and EC 267779 for maximum conservation period, while MS + 5 μM BA proved to be optimum for conserving the shoots of EC 99692 and EC 171284. Generally, the female shoot cultures of genotypes survived for longer period than male ones.

Keywords: clonal propagation; female; germplasm; male; Simmondsia chinensis; tissue culture
Subjects: auxins; clonal propagation; cultivar and genotype differences, micropropagation; cultivar and genotype differences, nodal explant culture in vitro; cytokinins; femal and male plants, jojoba; in vitro culture, cultivar differences, nodal explants; in vitro culture, from node and internode; in vitro culture, nutrient media modification; in vitro culture, root, rooting; jojoba, cultivar differences; male and female plants, jojoba; medicinal plants; node, callus; nutrient medium, Murashige and Skoog; Simmondsia chinensis

Published: March 1, 2004  Show citation

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Tyagi, R.K., & Prakash, S. (2004). Genotype- and Sex-Specific Protocols for in vitro Micropropagation and Medium-Term Conservation of Jojoba. Biologia plantarum, 48(1), 19-23. doi: 10.1023/B:BIOP.0000024270.02186.1f
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