biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:25-31, 2004 | DOI: 10.1023/B:BIOP.0000024271.38723.a6

Transformation of Indica Rice Through Particle Bombardment: Factors Influencing Transient Expression and Selection

K.B.R.S. Visarada1,*, N.P. Sarma1
1 Directorate of Rice Research, Rajendranagar, Hyderabad, India

Embryogenic, friable and small (ca. 3 mm) calli showed optimum gus expression and were best suited for selection during genetic transformation of rice through particle bombardment. Through prolonged culture of mature seeds on original callus induction medium, this type of calli could be produced in large numbers across several elite rice genotypes. To minimize the non-transformed escapes 50 mg dm-3 hygromycin and 8 mg dm-3 glufosinate ammonium were found to be critical during selection. Addition of selection marker during regeneration was essential. Regular and frequent (every 15 d) transfer of calli to fresh selection medium for three cycles was also important. A simple and economic procedure for screening large number of putative resistant plants was described.

Keywords: embryogenic calli; regeneration; selection
Subjects: embryogenic calli, Gus expression; β-glucuronidase; in vitro culture, from mature embryo and seed; in vitro culture, regeneration, organogenesis; nutrient medium, Murashige and Skoog; Oryza sativa; particle bombardment, rice; plasmid; rice genotypes, Gus expression; transformation, particle bombardment; transgenic plants; transient expression and selection

Published: March 1, 2004  Show citation

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Visarada, K.B.R.S., & Sarma, N.P. (2004). Transformation of Indica Rice Through Particle Bombardment: Factors Influencing Transient Expression and Selection. Biologia plantarum, 48(1), 25-31. doi: 10.1023/B:BIOP.0000024271.38723.a6
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