biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:33-39, 2004 | DOI: 10.1023/B:BIOP.0000024272.98338.5b

Changes in Antioxidative Protection in Bean Cotyledons During Natural and Continuous Irradiation-Accelerated Senescence

D. Procházková1, N. Wilhelmová1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 6, Czech Republic

We employed continuous irradiation (CL) for induction of premature senescence caused by enhanced production of reactive oxygen species. As a model plant we used bean (Phaseolus vulgaris L. cv. Jantar) cotyledons because they have well defined and a quite short life span. Senescence of bean cotyledons induced by CL progressed more rapidly than natural senescence: the life span of CL cotyledons was 13 d compared to 16 d in controls (C). Chl content was significantly lower in 10- and 13-d-old CL plants than in C plants and the change with age was not statistically significant. Activities of all antioxidative enzymes declined either with senescence onset or during whole life span. Activity of antioxidative enzymes, except ascorbate peroxidase, was lower in CL plants compared to C plants. On the contrary, contents of non-enzymatic antioxidants β-carotene and ascorbate were higher in CL plants than in C plants. No significant difference, except in the youngest cotyledons, was observed in glutathione content.

Keywords: antioxidants; Phaseolus vulgaris L.; reactive oxygen species; senescence
Subjects: antioxidants, protection of bean cotyledons, senescence; ascorbate peroxidase; carotenoids, antioxidants; carotenoids, cotyledon age and senescence; catalase; chlorophyll, antioxidants; chlorophyll, cotyledon senescence; cotyledons; fluorescence, chlorophyll, antioxidants; French bean, antioxidative protection during senescence; glutathione reductase; irradiation, continuous, accelerated senescence; Phaseolus vulgaris; photon flux density; premature senescence; reactive oxygen species (ROS); senescence, irradiation-accelerated, ROS; superoxide dismutase

Published: March 1, 2004  Show citation

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Procházková, D., & Wilhelmová, N. (2004). Changes in Antioxidative Protection in Bean Cotyledons During Natural and Continuous Irradiation-Accelerated Senescence. Biologia plantarum, 48(1), 33-39. doi: 10.1023/B:BIOP.0000024272.98338.5b
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