biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 48:41-48, 2004 | DOI: 10.1023/B:BIOP.0000024273.35976.cb

Antioxidant Enzyme Isoforms on Gels in Two Poplar Clones Differing in Sensitivity After Exposure to Ozone

R. Bernardi1, C. Nali2, P. Ginestri1, C. Pugliesi1,*, G. Lorenzini2, M. Durante1
1 Dipartimento di Biologia delle Piante Agrarie, Sezione di Genetica, Università degli Studi, Pisa, Italy
2 Dipartimento di Coltivazione e Difesa delle Specie Legnose "Giovanni Scaramuzzi", Università degli Studi, Pisa, Italy

The effect of acute ozone (O3) fumigation on isozyme patterns of superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX) in mature (ML) and young leaves (YL) of two poplar clones, contrasting in O3-sensitivity was analysed. Untreated leaves of both the O3-sensitive (O3-S) clone Eridano of Populus deltoides×P. maximowiczii and the O3-resistant (O3-R) clone I-214 of P.×euramericana showed four distinct SOD isoforms with a relative mobility (Rf) of 0.54 (MnSOD), 0.60 (Cu/ZnSOD), 0.65 (unidentified), and 0.71 (Cu/ZnSOD). After O3-fumigation the activity of the SOD isoforms showed only quantitative variations with respect to control plants. In ML of untreated O3-R plants seven POD isoforms (Rf= 0.13, 0.19, 0.34, 0.59, 0.64, 0.70 and 0.75) were found, while in YL one isoform (Rf= 0.34) was undetected. Only three POD isoforms in both ML and YL of untreated O3-S plants were resolved. The electrophoretic pattern of POD in O3-S leaves was greatly modified by acute O3-fumigation with the appearance of new isoforms in both YL and ML and the disappearance of an isoform (Rf= 0.13) in YL. Additionally, O3-exposure induced the appearance of two APX isoforms in YL (Rf= 0.66 and 0.70), and one isoform in ML (Rf= 0.70) of the O3-S clone. By contrast, the activity of the three APX isoformes (Rf= 0.64, 0.70 and 0.76) detected in O3-R leaves showed only quantitative variation with respect to untreated plants. From these data it is concluded that: 1) in these poplar hybrids antioxidant enzyme activity is developmentally regulated and greatly affected by acute O3 stress treatments and 2) the different enzymes activity displayed by the two poplar clones, especially for POD and APX isoformes, could partly explain their distinct O3-sensitivity.

Keywords: air pollution; antioxidant systems; oxidative stress; Populus deltoides×P. maximowiczii; Populus×euramericana
Subjects: antioxidants, enzyme isoforms; ascorbate peroxidase; clonal propagation; oxidative stress, sensitivity to ozone; ozone, antioxidant enzyme isoforms; peroxidase; poplar clones, oxidative stress, sensitivity to ozone; Populus × euramericana; Populus deltoides × P. maximowiczii; reactive oxygen species (ROS); ribulose-1,5-bisphosphate carboxylase/oxygenase; superoxide dismutase

Published: March 1, 2004  Show citation

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Bernardi, R., Nali, C., Ginestri, P., Pugliesi, C., Lorenzini, G., & Durante, M. (2004). Antioxidant Enzyme Isoforms on Gels in Two Poplar Clones Differing in Sensitivity After Exposure to Ozone. Biologia plantarum, 48(1), 41-48. doi: 10.1023/B:BIOP.0000024273.35976.cb
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