biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:73-79, 2004 | DOI: 10.1023/B:BIOP.0000024278.62419.ee

Effect of High Temperature on Protein Expression in Strawberry Plants

N.A. Ledesma1,*, S. Kawabata1, N. Sugiyama1
1 Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan

Strawberry plants (Fragaria×ananassa Duch.) cvs. Nyoho and Toyonoka were exposed to temperatures of 20, 33, and 42 °C for 4 h, and protein patterns in leaves and flowers was analyzed by 2-dimensional polyacrylamide gel electrophoresis and immunoblotting. In leaves and flowers of both cultivars, the content of most proteins decreased, but a few new proteins appeared in response to heat stress. These heat shock proteins (Hsps) were detected in the range of 19 - 29 kDa in leaves, and 16 - 26 kDa in flowers. The intensity of a 43 kDa protein spot increased in response to heat stress in Nyoho flowers, but not in Toyonoka flowers. The peaHsp17.7 antibody recognized one band at approximately 26 kDa in leaves, and two bands at approximately 16 and 17 kDa in flowers of both cultivars. These results show that the effects of heat stress on Hsp synthesis in strawberry plants differ between plant organs and between cultivars.

Keywords: Fragaria×ananassa; heat shock protein; immunoblotting
Subjects: cultivar and genotype differences, drought, heat shock protein; Fragaria × ananassa; heat-shock proteins; immunoblot; isoelectric focusing; protein expression, heat shock, flowering; strawberry, flowering, heat-shock protein; temperature, high, flowering, heat-shock protein

Published: March 1, 2004  Show citation

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Ledesma, N.A., Kawabata, S., & Sugiyama, N. (2004). Effect of High Temperature on Protein Expression in Strawberry Plants. Biologia plantarum, 48(1), 73-79. doi: 10.1023/B:BIOP.0000024278.62419.ee
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