biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:109-112, 2004 | DOI: 10.1023/B:BIOP.0000024284.84740.75

In vitro Shoot Bud Differentiation from Leaf Segments of Achras sapota

S.D. Purohit1,*, A. Singhvi1, R. Nagori1
1 Plant Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur-, India

Direct shoot bud differentiation was achieved in leaf segments of Achras sapota cv. Cricket Ball inoculated on Schenk and Hildebrandt's medium supplemented with 5.0 μM thidiazuron and 8.88 μM benzylaminopurine. Leaves from middle part of the shoots and segments obtained from middle portion of leaf showed highest potential to regenerate shoot buds. Histological examination of developing shoot buds showed their de novo regeneration with clear vascular connection with the mother tissues.

Keywords: adventitious shoot buds; benzylaminopurine; regeneration; thidiazuron; tissue culture
Subjects: Achras sapota (zapota); adventitious shoot; auxins; cytokinins; in vitro culture, from leaf segments; in vitro culture, from shoot-tip, bud; in vitro culture, nutrient media modification; in vitro culture, regeneration, organogenesis; nutrient medium, SH (Schenk and Hildebrandt); shoot proliferation; thidiazuron

Published: March 1, 2004  Show citation

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Purohit, S.D., Singhvi, A., & Nagori, R. (2004). In vitro Shoot Bud Differentiation from Leaf Segments of Achras sapota. Biologia plantarum, 48(1), 109-112. doi: 10.1023/B:BIOP.0000024284.84740.75
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    References

    1. Arockiasamy, S., Prakash, S., Ignacimuthu, S.: Direct organogenesis from mature leaf and petiole explants of Eryngium foetidum L.-Biol. Plant. 45: 129-132, 2002. Go to original source...
    2. Bapat, V.A., Narayanaswamy, S.: Mesocarp and endosperm culture of Achras sapota Linn. in vitro.-Indian J. exp. Biol. 15: 294-296, 1977.
    3. Caboni, E., Tonelli, M.G.: Effect of 1,2-benzioxazole-3-acetic acid on adventitious shoot regeneration and in vitro rooting in apple.-Plant Cell Rep. 18: 985-988, 1999. Go to original source...
    4. Ellis, D.D., Barczynska, H., McCown, B.H., Nelson, N.: A comparison of BA, zeatin and thidiazuron for adventitious bud formation from Picea glauca embryos and epicotyl explants.-Plant Cell Tissue Organ Cult. 27: 281-287, 1991. Go to original source...
    5. Escalettes, V., Dosba, F.: In vitro adventitious shoot regeneration from leaves of Prunus spp.-Plant Sci. 90: 201-209, 1993. Go to original source...
    6. Fasolo, F., Zimmerman, R.H., Fordham, I.: Adventitious shoot formation on excised leaves of in vitro grown shoots of apple cultivars.-Plant Cell Tissue Organ. Cult. 16: 75-87, 1989. Go to original source...
    7. Fiola, J.A., Hassan, M.A., Swartz, H.J., Bors, T.H., McNicols, T.: Effect of thidiazuron, light fluence rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledons and leaves.-Plant Cell Tissue Organ Cult. 20: 223-228, 1990.
    8. Haccius, B.: Question of unicellular origin of non-zygotic embryos in callus cultures.-Phytomorphology 28: 74-81, 1978.
    9. Huetteman, C.A., Preece, J.E.: Thidiazuron: a potent cytokinin for woody plant tissue culture.-Plant Cell Tissue Organ Cult. 33: 105-119, 1993. Go to original source...
    10. Khurana, P., Khurana, J.: Application of genetic transformation to tree biotechnology.-Indian J. exp. Biol. 37: 627-638, 1999.
    11. Litz, R.E.: In vitro strategies for tropical tree improvement.-In: Dhawan, V. (ed.): Applications of Biotechnology in Forestry and Horticulture. Pp. 109-118. Plenum Press, New York 1989. Go to original source...
    12. Mishra, P.: Direct differentiation of shoot buds from leaf explants of Cajanus cajan L.-Biol. Plant. 45: 347-351, 2002. Go to original source...
    13. Purohit, S.D., Singhvi, A.: Micropropagation of Achras sapota through enhanced axillary branching.-Scientia Hort. 76: 219-229, 1998. Go to original source...
    14. Sachdeva, S., Mehra, P.N.: In vitro studies on Achras sapota (Chikoo): callus and early morphogenesis.-Phytomorphology 36: 315-324, 1986.
    15. Schenk, R.V., Hildebrandt, A.C.: Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures.-Can. J. Bot. 50: 199-204, 1972.
    16. Tanimoto, S., Harada, H.: Effects of cytokinins and anticytokinin on the initial stage of adventitious bud differentiation in the epidermis of Torenia stem segments.-Plant Cell Physiol. 23: 1371-1376, 1982.

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