Biologia plantarum 48:113-115, 2004 | DOI: 10.1023/B:BIOP.0000024285.44356.99
Detection of Intra-Clonal Genetic Variability in Vegetatively Propagated Tea Using RAPD Markers
- 1 Division of Biotechnology, Institute of Himalayan Bioresource Technology, Palampur (HP), India
The role of random amplified polymorphic DNA (RAPD) markers in detecting intra-clonal genetic variability in vegetatively propagated UPASI-9 clone of tea (Camellia sinensis) was studied. Twenty five decamer primers were used, of which three did not amplify, three gave single bands and the rest of nineteen primers generated upto twelve bands (an average of 6.3 bands per primer). Twenty one primers exhibiting amplified products gave monomorphic banding patterns. Only one primer (OPE-17) gave a unique extra band of similar size in four plants.
Keywords: Camellia sinensis; polymerase chain reaction (PCR)
Subjects: Camellia sinensis; clonal propagation; in vitro culture, micropropagation; intraclonal genetic variability; markers, RAPD; polymerase chain reaction (PCR); random amplified polymorphic DNA (RAPD) markers; tea, vegetative propagation
Published: March 1, 2004 Show citation
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Singh, M., Saroop, J., & Dhiman, B. (2004). Detection of Intra-Clonal Genetic Variability in Vegetatively Propagated Tea Using RAPD Markers. Biologia plantarum, 48(1), 113-115. doi: 10.1023/B:BIOP.0000024285.44356.99
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