biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:125-128, 2004 | DOI: 10.1023/B:BIOP.0000024288.82679.50

Glutamine: A Suitable Nitrogen Source for Enhanced Shoot Multiplication in Cucumis sativus L.

A. Vasudevan1, N. Selvaraj2, A. Ganapathi1,*, S. Kasthurirengan1, V. Ramesh Anbazhagan1, M. Manickavasagam1
1 Department of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
2 Department of Botany, Periyar E.V.R. College (Autonomous), Tiruchirappalli, Tamil Nadu, India

Shoot tip explants of cucumber (Cucumis sativus L. cv. Poinsett 76) were cultured in vitro on Murashige-Skoog medium with L-glutamine, ammonium nitrate, adenine sulphate, asparagine, ammonium succinate, potassium nitrate and sodium nitrate as the nitrogen sources along with optimal concentration of 0.044 mM benzyladenine to study their effects on in vitro morphogenesis. The explants grown with 0.068 mM L-glutamine displayed the highest culture response (74.6 %) and greatest shoot number per explant (13.6) at the end of two subcultures. The explants cultured with other nitrogen sources resulted in low culture frequency and low number of shoots per explant accompanied by basal callusing and necrosis.

Keywords: benzyladenine; cucumber; shoot tip explants
Subjects: amino acids; cucumber, glutamione, N source; Cucumis sativus; glutamine, enhanced shoot multiplication; in vitro culture, from shoot-tip, bud; in vitro culture, nutrient media modification; nitrogen source, glutamine; nutrient medium, Murashige and Skoog

Published: March 1, 2004  Show citation

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Vasudevan, A., Selvaraj, N., Ganapathi, A., Kasthurirengan, S., Ramesh Anbazhagan, V., & Manickavasagam, M. (2004). Glutamine: A Suitable Nitrogen Source for Enhanced Shoot Multiplication in Cucumis sativus L. Biologia plantarum, 48(1), 125-128. doi: 10.1023/B:BIOP.0000024288.82679.50
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