biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:205-209, 2004 | DOI: 10.1023/B:BIOP.0000033446.43495.0c

Estimation of Genetic Variability of Vigna radiata Cultivars by RAPD Analysis

S. Betal1, P. Roy Chowdhury2, S. Kundu1, S. Sen Raychaudhuri1,*
1 Department of Biophysics and Molecular Biology, University of Calcutta, Calcutta, India
2 BKC College, Calcutta, India

DNA was isolated from 14 cultivars of Vigna radiata (L.) Wilczek and subjected to RAPD analysis using 14 random decamer primers. These cultivars revealed polymorphism with respect to RAPD markers and were subjected to hierarchical cluster analysis. A dendrogram was prepared based on these data. Analysis of banding patterns confirmed that two strongly aromatic cultivars IC1, IC4, were closely linked. But another aromatic cultivar, B1, formed a separate cluster. The high yielding cultivars were closely related to B1. The phylogenetic tree constructed by the neighbour joining method showed that RAPD results were correlated with morphological characters like plant height, leaf and seed size, seed colour, etc.

Keywords: dendrogram; mungbean; phylogenetic tree; random amplified polymorphic DNA
Subjects: cultivar and genotype differences, DNA isolation; dendrogram, phylogenetic tree; genetic variability estimation; mung bean, genetic variability; phylogenetic tree, dendrogram; random amplified polymorphic DNA (RAPD) markers; Vigna radiata

Published: June 1, 2004  Show citation

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Betal, S., Roy Chowdhury, P., Kundu, S., & Sen Raychaudhuri, S. (2004). Estimation of Genetic Variability of Vigna radiata Cultivars by RAPD Analysis. Biologia plantarum, 48(2), 205-209. doi: 10.1023/B:BIOP.0000033446.43495.0c
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