biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:227-235, 2004 | DOI: 10.1023/B:BIOP.0000033449.19251.8a

Diurnal and Seasonal Modulation of Sucrose Phosphate Synthase Activity in Leaves of Prosopis juliflora

U.V. Pathre1,*, A.K. Sinha1, P.A. Shirke1, S.A. Ranade1
1 National Botanical Research Institute, Lucknow, India

Diurnal changes of sucrose-phosphate synthase (SPS) activity in different seasons were measured in Prosopis juliflora (Swartz) DC. leaves. SPS activity showed large variations with two distinct peaks, one around 09:00 and another at 21:00. The diurnal pattern was apparently not due to circadian rhythm since the activities were directly related to the changes in environmental parameters (irradiance, temperature, and leaf to air vapour pressure deficit) in different seasons. During the day, the enzyme showed changes in kinetic properties, differential sensitivity to allosteric modulators, differential response to ATP concentration, to concentration of endogenous sucrose, and to protein phosphatase inhibitors. These results taken together indicate the modulation of SPS in synchrony with photosynthesis and suggest the existence of multiple levels of modulation, presumably as an adaptive response to changing environmental extremes.

Keywords: allosteric modulation; photosynthesis; phosphorylation, dephosphorylation; sucrose synthesis
Subjects: allosteric modulation; diurnal changes, sucrose phosphate synthase, gas exchange; fructose-6-phosphate; gas exchange, sucrose phosphate synthase; glucose-6-phosphate; N-morpholinopropanesulphonic acid; phosphoglucoisomerase; phosphorylation/dephosphorylation sucrose phosphate synthase; photon flux density; Prosopis juniflora; seasonal changes, sucrose phosphate synthase, gas exchange; starch, diurnal changes; sucrose phosphate synthase; sucrose synthesis

Published: June 1, 2004  Show citation

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Pathre, U.V., Sinha, A.K., Shirke, P.A., & Ranade, S.A. (2004). Diurnal and Seasonal Modulation of Sucrose Phosphate Synthase Activity in Leaves of Prosopis juliflora. Biologia plantarum, 48(2), 227-235. doi: 10.1023/B:BIOP.0000033449.19251.8a
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