biologia plantarum

Biologia plantarum 48:289-292, 2004 | DOI: 10.1023/B:BIOP.0000033458.88441.67

Micropropagation of Sesbania rostrata from the Cotyledonary Node

A.K. Jha¹, S. Prakash¹, N. Jain^1,*, K. Nanda², S.C. Gupta¹

¹ Department of Botany, University of Delhi, Delhi, India

² Department of Botany, Daulat Ram College, University of Delhi, Delhi, India

Multiple shoots were induced from the cotyledonary nodes derived from seedling of Sesbania rostrata on Nitsch (1969; N) medium supplemented with various concentrations of benzyladenine (BA). 1 mg dm^-3 BA proved to be the best, eliciting 5.8 ± 1.0 shoots per explant in 100 % cultures. The elongation of shoots was best at 2.0 mg dm^-3 BA. The shoot proliferation capacity increased to 7.5 shoots per explant following transfer of explants to the fresh shoot multiplication medium (MS + 1.0 mg dm^-3 BA), after an initial incubation of 30 d. To further enhance number of shoots per explant an alternative strategy of cultivation of mother explant on fresh shoot multiplication medium after excision of shoots was adopted. Following the repeated harvesting of shoots an average of 33 shoots per explant could be obtained. The in vitro regenerated shoots produced roots when transferred to half-strength MS medium supplemented with 3 % sucrose and 1 mg dm^-3 IBA. The developed plantlets were planted in the soil and transferred to the field after an acclimatization period of 3 - 4 months. These plants produced flowers and fruits in the field and exhibited the development of prominent and more organized stem nodules as compared to the in vivo raised plants of the same age.

Keywords: auxins; cytokinins; shoot proliferation; stem nodule; tissue culture

Subjects: cultivar and genotype differences, nodal explant culture in vitro; in vitro culture, from node and internode; in vitro culture, micropropagation; node, callus; nutrient medium, Murashige and Skoog; Sesbania rostrata; shoot proliferation

Published: June 1, 2004  Show citation