biologia plantarum

Biologia plantarum 48:293-296, 2004 | DOI: 10.1023/B:BIOP.0000033459.48057.8b

Detection of Proteins Possibly Involved in Self-Incompatibility Response in Distylous Buckwheat

J. Miljuš-Đukić¹, S. Ninković², S. Radović³, V. Maksimović¹, J. Brkljačić¹, M. Nešković²

¹ Institute of Molecular Genetics and Genetic Engineering, Belgrade

² Institute for Biological Research "S, Belgrade

³ Faculty of Biological Sciences, Belgrade

Buckwheat (Fagopyrum esculentum Moench) is a heterostylous plant displaying heteromorphic sporophytic self-incompatibility (SI). In order to detect proteins involved in SI, pistils from both long and short styles were isolated and then selfed or cross-pollinated. One-dimensional gel electrophoresis revealed that short pistils 2 h after selfing contained an unique 50 kDa protein. In the two-dimensional electrophoresis two distinct groups of proteins possibly involved in SI response were detected in the short, and one in the long pistils.

Keywords: Fagopyrum esculentum; heteromorphic self-incompatibility

Subjects: buckwheat, heteromorphic self-incompatibility; Fagopyrum aesculentum; heteromorphic self-incompatibility; protein detection, sporophytic self-incompatibility

Published: June 1, 2004  Show citation