biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:313-316, 2004 | DOI: 10.1023/B:BIOP.0000033464.36078.24

Regeneration Capacity of Calli Derived from Immature Embryos in Spring Barley Cultivars

E. Halámková1,*, J. Vagera1,2, L. Ohnoutková1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic
2 Faculty of Sciences, Palacký University, Olomouc, Czech Republic

Callogenesis, somatic embryogenesis and regeneration capacity in twenty-three agronomically important spring barley (Hordeum vulgare L.) cultivars on induction media with 2,4-dichlorophenoxyacetic acid (2,4-D) or 3,6-dichloro-o-anisic acid (dicamba) and on modified regeneration media were studied. The frequency of zygotic embryos exhibiting callogenesis varied from 88 to 100 % according to genotype. Dicamba was more suitable for somatic embryogenesis induction and exhibited a higher frequency of regenerants than did 2,4-D. Green regenerants were obtained in all cultivars, and there were no albino plants. Except for cv. Victor all cultivars used in the experiment showed lower regeneration capacity as compared to the model cv. Golden Promise.

Keywords: callus induction; dicamba; 2,4-dichlorophenoxyacetic acid; Hordeum vulgare; in vitro culture; somatic embryogenesis
Subjects: barley, immature embryos; cultivar and genotype differences, immature embryos; 3,6-dichloro-o-anisic acid; embryo, immature; Hordeum vulgare; immature embryos; in vitro culture, callus induction; in vitro culture, from immature embryos; in vitro culture, nutrient media modification; in vitro culture, regeneration, organogenesis; in vitro culture, somatic embryogenesis

Published: June 1, 2004  Show citation

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Halámková, E., Vagera, J., & Ohnoutková, L. (2004). Regeneration Capacity of Calli Derived from Immature Embryos in Spring Barley Cultivars. Biologia plantarum, 48(2), 313-316. doi: 10.1023/B:BIOP.0000033464.36078.24
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