biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:333-338, 2004 | DOI: 10.1023/B:BIOP.0000041083.41294.31

In vitro Regeneration and Transformation of Blackstonia perfoliata

A. Bijelović1, N. Rosić2, J. Miljuš-Djukić3, S. Ninković2, D. Grubišić2
1 Institute of Botany, Faculty of Biology, Belgrade, Serbia and Montenegro, India e-mail
2 Institute for Biological Research "Siniša Stanković", 29, Belgrade, Serbia and Montenegro, India
3 Institute for Molecular Genetics and Genetic Engineering, Belgrade, Serbia and Montenegro, India

In vitro root culture of yellow wort (Blackstonia perfoliata (L.) Huds.) was initiated on Murashige and Skoog (MS) medium. In the presence of benzylaminopurine (BAP) numerous adventitious buds formed, which developed into shoots. Presence of indole-3-butyric acid (IBA) in media significantly decreased number of buds, but increased development of lateral roots. On hormone-free medium shoots successfully rooted and developed flowers and viable seeds that formed another generation. Shoot cultures of B. perfoliata inoculated with suspension of Agrobacterium rhizogenes strain A4M70GUS developed hairy roots at 3 weeks and they were cultured on hormone-free MS medium. Spontaneous shoot regeneration occurred in 3 clones.

Keywords: Agrobacterium rhizogenes; hairy roots; regeneration; root culture
Subjects: Agrobacterium-mediated transformation; Agrobacterium rhizogenes; auxins; Blackstonia perfoliata; cytokinins; hairy root; in vitro culture, nutrient media modification; in vitro culture, regeneration, organogenesis; in vitro culture, root, rooting; medicinal plants; nutrient medium, Murashige and Skoog; root, hairs; transformation, Agrobacterium-mediated; transgenic plants; yellow wort, regeneration, transformation

Published: September 1, 2004  Show citation

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Bijelović, A., Rosić, N., Miljuš-Djukić, J., Ninković, S., & Grubišić, D. (2004). In vitro Regeneration and Transformation of Blackstonia perfoliata. Biologia plantarum, 48(3), 333-338. doi: 10.1023/B:BIOP.0000041083.41294.31
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