biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 48:345-350, 2004 | DOI: 10.1023/B:BIOP.0000041085.82296.9c

Cloning of a Gene Encoding a Putative Ethylene Receptor in Catharanthus roseus and its Expression in Plant and Cell Cultures

N. Papon1, M.M. Senoussi1, F. Andreu1, M. Rideau1, J.-C. Chenieux1, J. Creche1
1 Department of Plant Molecular and Cell Biology, Faculty of Pharmacy, France

A full length cDNA (designated CrETR1) was isolated by polymerase chain reaction amplification of a cDNA library from periwinkle (Catharanthus roseus) cell cultures. CrETR1 cDNA encodes a polypeptide of 740 amino acids with a predicted molecular mass of 82 kDa. The deduced protein contains a hydrophobic ethylene-binding transmembrane region, a GAF domain, a third domain homologous to the histidine protein kinase domain of the prokaryotic two-component systems, and a fourth carboxyl-terminal domain homologous to the receiver domain of the response regulators, as found in the A. thaliana ethylene receptor ETR1. CrETR1 transcripts are strongly accumulated in petals and ovaries of C. roseus young plants whereas no significant changes are detected in cell cultures submitted to various stress or hormonal (including ethylene) treatments. The amount of the monoterpene indole alkaloid ajmalicine in the cells treated by ethylene is reduced after addition of inhibitors of histidine kinases showing a possible involvement of CrETR1 protein in the ethylene-related signalling pathway leading to alkaloid biosynthesis enhancement in C. roseus cell cultures.

Keywords: cell culture; histidine kinase; Madagascar periwinkle; salicylanilides
Subjects: Arabidopsis thaliana; Brassica oleracea; Catharanthus roseus; Cucumis melo var. reticulatus; dendrogram, phylogenetic tree; ethylene receptor, gene encoding; Fragaria × ananassa; gene cloning, ethylene receptor expression; histidine kinase; in vitro culture, cell culture; Madagascar periwincle, cell culture; Malus domestica; Mangifera indica; Nicotiana tabacum; nutrient medium, B5 (Gamborg); Passiflora edulis; Pelargonium × hortorum; periwincle, cell culture; Petunia × hybrida; phylogenetic tree, dendrogram; polymerase chain reaction (PCR); Prunus persica; Pyrus communis; salicylanilides, cell culture; Vitis vinifera

Published: September 1, 2004  Show citation

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Papon, N., Senoussi, M.M., Andreu, F., Rideau, M., Chenieux, J.-C., & Creche, J. (2004). Cloning of a Gene Encoding a Putative Ethylene Receptor in Catharanthus roseus and its Expression in Plant and Cell Cultures. Biologia plantarum, 48(3), 345-350. doi: 10.1023/B:BIOP.0000041085.82296.9c
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