biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:361-366, 2004 | DOI: 10.1023/B:BIOP.0000041087.17353.d8

Characterization of Transgenic Rice Plants Expressing an Arabidopsis FAD7

J. Song1, D.E. Lee1, S. Jung1, H. Kim1, O. Han1, B.H. Cho2, I.J. Lee3, K. Back1
1 Department of Biotechnology, Agricultural Plant Stress Research Center, Chonnam National University, Gwangju, Korea
2 Division of Applied Plant Science, Chonnam National University, Gwangju, Korea
3 Division of Applied Plant Science, Kyungpook National University, Taegu, Korea

Fatty acid ω-3 desaturase (FAD) is the key enzyme catalyzing the formation of trienoic fatty acids. We utilized an Arabidopsis FAD7 gene and the seven independent transgenic rice plants harbouring 1 to 3 copies of this gene were generated. The expression of FAD7 mRNA was different among independent transgenic lines regardless of the copy number. The total linolenic acid (18:3) contents reduced by about 7 - 32 % in transgenic rice plants but the linoleic acid (18:2) content increased accordingly. With or without wounding treatments, the jasmonate content was higher in transgenic lines than in wild-type rice plant. The transgenic lines overproducing jasmonate also showed increased expression of PR1b mRNA and allene oxide synthase inresponse to wounding.

Keywords: fatty acid desaturase; jasmonic acid; linoleic acid; linolenic acid; malondialdehyde
Subjects: Agrobacterium-mediated transformation; Agrobacterium tumefaciens; fatty acid desaturase (FAD); fatty acids; jasmonates, jasmonic acid; linoleic acid; linolenic acid; malonyldialdehyde; medicinal plants; rice, transgenic; transformation, Agrobacterium-mediated; transgenic plants

Published: September 1, 2004  Show citation

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Song, J., Lee, D.E., Jung, S., Kim, H., Han, O., Cho, B.H., Lee, I.J., & Back, K. (2004). Characterization of Transgenic Rice Plants Expressing an Arabidopsis FAD7. Biologia plantarum, 48(3), 361-366. doi: 10.1023/B:BIOP.0000041087.17353.d8
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