biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:389-394, 2004 | DOI: 10.1023/B:BIOP.0000041091.03068.ee

Heat Shock Induces Programmed Cell Death in Wheat Leaves

T. Fan1, T. Xing2
1 Agriculture and Agri-Food Canada, Cereal Research Centre, MB, Canada
2 Department of Biology and Institute of Biochemistry, Carleton University, ON, Canada, e-mail

Programmed cell death (PCD) was triggered in wheat leaves by a heat treatment (42 °C). This treatment caused DNA fragmentation as shown in DNA laddering analysis and in terminal deoxynucleotidyl transferase-mediated deoxyuridine-5'-triphosphate (dUTP) nick end labeling (TUNEL) analysis. Methanol and acetone treatment of leaves significantly blocked PCD. Western analysis indicated that a 65 kDa poly(ADP-ribose) polymerase-like protein was degraded during the treatment. However, high temperature (80 °C) treatment caused necrosis but not PCD.

Keywords: caspase-like protease; DNA ladder; necrosis; PARP; TUNEL
Subjects: caspase-like protease; cell death, programmed; DNA ladder; poly(ADP-ribose) polymerases; programmed cell death; temperature, high, cell death induction; wheat, programmed cell death

Published: September 1, 2004  Show citation

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Fan, T., & Xing, T. (2004). Heat Shock Induces Programmed Cell Death in Wheat Leaves. Biologia plantarum, 48(3), 389-394. doi: 10.1023/B:BIOP.0000041091.03068.ee
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    References

    1. Amé, J., Rolli, V., Schreiber, V., Niedergang, C., Apiou, F., Decker, P., Muller, S., Höger, T., Murcia, J.M., and de Murcia, G.J.: PARP-2, a novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase.-Biol. Chem.274: 17860-17868, 1999. Go to original source...
    2. Babiychuk, E., Cottrill, P.B., Storozhenko, S., Fuangthong, M., Chen, Y., O'Farrell, M., Van Montagu, M., Inzé, D., Kushnir, S.: Higher plants possess two structurally different poly(ADP-ribose) polymerases.-Plant J. 15: 635-645, 1998. Go to original source...
    3. Balk, J., Leaver, C.J., McCabe, P.F.: Translocation of cytochrome c from the mitochondria to the cytosol occurs during heat-induced programmed cell death in cucumber plants.-FEBS Lett. 463: 151-154, 1999. Go to original source...
    4. Danon, A., Delorme, V., Mailhac, N., Gallois, P.: Plant programmed cell death: a common way to die.-Plant Physiol. Biochem. 388: 647-655, 2000. Go to original source...
    5. Danon, A., Gallois, P.: UV-C radiation induces apoptotic-like changes in Arabidopsis thaliana.-FEBS Lett. 437: 131-136, 1998. Go to original source...
    6. De Murcia, G., Ménissier de Murcia, J.: Poly(ADP-ribose) polymerase: a molecular nick-sensor.-Trends Biochem.Sci. 19: 172-176, 1994. Go to original source...
    7. Doucet-Chabeaud, G., Godon, C., Brutesco, C., De Murcia, G., Kazmaier, M.: Ionising radiation induces the expression of PARP-1 and PARP-2 genes in Arabidopsis.-Mol. genet. Genomics 265: 945-963, 2001. Go to original source...
    8. Elbaz, M., Avni, A., Weil, M.: Constitutive caspase-like machinery executes programmed cell death in plant cells.-Cell Death Differ. 9: 726-733, 2002. Go to original source...
    9. Fan, T., Lu, H., Hu, H., Shi, L., McClarty, G.A., Nance, D.M., Greenberg, A.H., Zhong, G.: Inhibition of apoptosis in chlamydia-infected cells: blockade of mitochondrial cytochrome c release and caspase activation.-J. exp. Med.4: 487-496, 1998. Go to original source...
    10. Gilchrist, D., Lincoln, J., Richeal, C., Pan, Z., Overduin, B., Fan, X., Smith, K., Li, Juan, Haworth, R., Bostock, R.: Biology of Plant-Microbe Interactions. Vol. 2.-International Society for Molecular Plant-Microbe Interactions, St. Paul 2000.
    11. Greenberg, J.T.: Programmed cell death: a way of life for plants.-Proc. nat. Acad. Sci. USA 93: 12094-12097, 1996. Go to original source...
    12. Jones, A.M.: Programmed cell death in development and defense.-Plant Physiol. 125: 94-97, 2001. Go to original source...
    13. Jones, A.: Does the plant mitochondrion integrate cellular stress and regulate programmed cell death?-Trends Plant Sci. 5: 225-230, 2002. Go to original source...
    14. Korthout, H., Berecki, G., Bruin, W., Van Duijn, B., Wang, M.: The presence and subcellular localization of caspase 3-like proteinases in plant cells.-FEBS Lett. 475: 139-144, 2002. Go to original source...
    15. Koukalová, B., Kovařík, A., Fajkus, J., ©iroký, J.: Chromatin fragmentation associated with apoptotic changes in tobacco cells exposed to cold stress.-FEBS Lett. 414: 289-292, 1997. Go to original source...
    16. Kuriyama, H., Fukuda, H.: Developmental programmed cell death in plants.-Curr. Opinion Plant Biol. 5: 568-573, 2002. Go to original source...
    17. Lam, E., Del Pozo, O.: Caspase-like protease involvement in the control of plant cell death.-Plant mol. Biol. 44: 417-428, 2000. Go to original source...
    18. Lam, E., Kota, N., Lawton, M.: Programmed cell death, mitochondria and the plant hypersensitive response.-Nature 411: 848-853, 2001. Go to original source...
    19. Lam, E., Pontier, D., Del-Pozo, O.: Die and let live-programmed cell death in plants.-Curr. Opinion Plant Biol. 2: 502-507, 1999. Go to original source...
    20. Lazebnik, Y.A., Kaufmann, S.H., Desnoyers, S., Poirier, G.G., Earnshaw, W.C.: Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE.-Nature 371: 346-347, 1994. Go to original source...
    21. Lepiniec, L., Babiychuk, E., Kushnir, S., Van Montagu, M., Inzé, D.: Characterization of an Arabidopsis thaliana cDNA homologue to animal poly(ADP-ribose) polymerase.-FEBS Lett. 364: 103-108, 1995. Go to original source...
    22. Lindahl, T., Satoh, M.S., Poirier, G.G., Klungland, A.: post-translational modification of poly(ADP-ribose) polymerase induced by DNA strand breaks.-Trends Biochem. Sci. 20: 405-411, 1995. Go to original source...
    23. Nicholson, D.W., Ali, A., Thornberry, N.A., Vaillancourt, J.P., Ding, C.K., Gallant, M., Gareau, Y., Griffin, P.R., Labelle, M., Lazebnik, Y.A., Munday, N.A., Raju, S.M., Smulson, M.E., Yamin, T., Yu, V.L., Miller, D.K.: Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis.-Nature 376: 37-43, 1995. Go to original source...
    24. Ning, S., Wang, L., Song, Y.: Identification of programmed cell death in situ in individual plant cell in vivo using a chromosome preparation technique.-J. exp. Bot. 369: 651-658, 2002. Go to original source...
    25. Richberg, M.H., Aviv, D.H., Dangl, J.L.: Dead cells do tell tales.-Curr. Opinion Plant Biol. 1: 480-485, 1998. Go to original source...
    26. Sun, Y., Zhao, Y., Hong, X., Zhai, Z.: Cytochrome c release and caspase activation during menadione-induced apoptosis in plants.-FEBS Lett. 462: 317-321, 1999. Go to original source...
    27. Swidzinski, J.A., Sweetlove, L.J., Leaver, C.J.: A custom microarray analysis of gene expression during programmed cell death in Arabidopsis thaliana.-Plant J. 30: 431-446, 2002. Go to original source...
    28. Tewari, M., Quan, L.T., Rourke, K., Desnoyers, S., Zeng, Z., Beidler, D.R., Poirier, G.G., Salvesen, G.S., Dixit, V.M.: Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase.-Cell 81: 801-809, 1995. Go to original source...
    29. Wang, H., Li, J., Bostock, R.M., Gilchrist, D.G.: Apoptosis: a functional paradigm for programmed plant cell death induced by a host-selective phytotoxin and invoked during development.-Plant Cell 8: 375-391, 1996. Go to original source...
    30. Wyllie, A.H.: Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation.-Nature 284: 555-556, 1980. Go to original source...
    31. Wyllie, A.H., Kerr, F.F.R., Currie, A.R.: Cell death: the significance of apoptosis.-Int. rev. Cytol. 68: 251-306, 1980. Go to original source...

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