biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:425-430, 2004 | DOI: 10.1023/B:BIOP.0000041097.03177.2d

Jasmonic Acid and Salicylic Acid Induce Accumulation of β-1,3-Glucanase and Thaumatin-Like Proteins in Wheat and Enhance Resistance Against Stagonospora nodorum

J. Jayaraj1, S. Muthukrishnan1, G.H. Liang2, R. Velazhahan3
1 Department of Biochemistry, Kansas State University, Manhattan, USA
2 Department of Agronomy, Kansas State University, Manhattan, USA
3 Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Tamil Nadu, India, e-mail

The effect of application of jasmonic acid (JA) and salicylic acid (SA) on the induction of resistance in wheat to Stagonospora nodorum and on the induction of μ-1,3-glucanase and thaumatin-like proteins (TLPs) was studied. Western blot analysis revealed that two β-1,3-glucanases with apparent molecular masses of 31 and 33 kDa that cross-reacted with a barley glucanase antiserum were induced in wheat leaves after treatment with JA and SA. When wheat plants were treated with SA and JA, a TLP with an apparent molecular mass of 25 kDa and several other isoforms of TLP were induced. Pre-treatment of wheat plants with SA and JA significantly reduced (up to 56 %) the incidence of leaf blotch disease incited by S. nodorum compared with untreated control plants.

Keywords: leaf blotch disease; pathogenesis-related protein; systemic acquired resistance; Triticum aestivum
Subjects: β-1,3-glucanase; jasmonates, jasmonic acid; leaf blotch disease; pathogenesis-related (PR) protein; protein, thaumatin-like; resistance against Stagonospora nodorum; salicylic acid; systemic acquired resistance; thaumatin-like proteins; Triticum aestivum; wheat, thaumatin-like proteins

Published: September 1, 2004  Show citation

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Jayaraj, J., Muthukrishnan, S., Liang, G.H., & Velazhahan, R. (2004). Jasmonic Acid and Salicylic Acid Induce Accumulation of β-1,3-Glucanase and Thaumatin-Like Proteins in Wheat and Enhance Resistance Against Stagonospora nodorum. Biologia plantarum, 48(3), 425-430. doi: 10.1023/B:BIOP.0000041097.03177.2d
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    References

    1. Anderson, J.M.: Membrane-derived fatty acids as precursors to second messengers.-In: Liss, A.R. (ed.): Second Messengers in Plant Growth and Development. Pp. 181-212. Alan R. Liss Inc., New York 1989.
    2. Anguelova, V.S., Van der Westhuizen, A.J., Pretorius, Z.A.: Intercellular proteins and β-1,3-glucanase activity associated with leaf rust resistance in wheat.-Physiol. Plant. 106: 393-401, 1999. Go to original source...
    3. Chen, W.P., Chen, P.D., Liu, D.J., Kynast, R., Friebe, B., Velazhahan, R., Muthukrishnan, S., Gill, B.S.: Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene.-Theor. appl. Genet. 99: 755-760, 1999. Go to original source...
    4. Cohen, Y., Gisi, U., Niederman, T.: Local and systemic protection against Phytophthora infestans induced in potato and tomato plants by jasmonic acid and jasmonic acid methyl ester.-Phytopathology 83: 1054-1062, 1993. Go to original source...
    5. Datta, K., Velazhahan, R., Oliva, N., Ona, I., Mew, T., Khush, G.S., Muthukrishnan, S., Datta, S.K.: Overexpression of cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmentally friendly resistance to Rhizoctonia solani causing sheath blight disease.-Theor. appl. Genet. 98: 1138-1145, 1999. Go to original source...
    6. Davis, J.M., Wu, H.G., Cooke, J.E.K., Reed, J.M., Luce, K.S., Michler, C.H.: Pathogen challenge, salicylic acid, and jasmonic acid regulate expression of chitinase gene homologs in pine.-Mol. Plant-Microbe Interact. 15: 380-387, 2002. Go to original source...
    7. Delaney, T.P., Friedrich, L., Ryals, J.: Arabidopsis signal transduction mutant defective in chemically and biologically induced disease resistance.-Proc. nat. Acad. Sci. USA 92: 6602-6606, 1995. Go to original source...
    8. Farmer, E.E., Weber, H., Vollenweider, S.: Fatty acid signaling in Arabidopsis.-Planta 206: 167-174, 1998. Go to original source...
    9. Gaffney, T., Friedrich, L., Vernooij, B., Negrotto, D., Nye, G., Uknes, S., Ward, E., Kessmann, H., Ryals, J.: Requirement of salicylic acid for the induction of systemic acquired resistance.-Science 261: 754-756, 1993. Go to original source...
    10. Ham, K.S., Kauffmann, S., Albersheim, P., Darvill, A.G.: Host-pathogen interactions. XXXIX. A soybean pathogenesis-related protein with β-1,3-glucanase activity releases phytoalexin elicitor-active heat-stable fragments from fungal walls.-Mol. Plant-Microbe Interact. 4: 545-552, 1991. Go to original source...
    11. Hammerschmidt, R.: Induced disease resistance: How do induced plants stop pathogens?-Physiol. mol. Plant Pathol. 55: 77-84, 1999. Go to original source...
    12. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 277: 680-684, 1970. Go to original source...
    13. Lawrence, C.B., Joosten, M.H.A.J., Tuzun, S.: Differential induction of pathogenesis-related proteins in tomato by Alternaria solani and the association of a basic chitinase isozyme with resistance.-Physiol. mol. Plant Pathol. 48: 361-377, 1996. Go to original source...
    14. Lawton, K.A., Beck, J., Potter, S., Ward, E., Ryals, J.: Regulation of cucumber class III chitinase gene expression.-Mol. Plant-Microbe Interact. 7: 48-57, 1994. Go to original source...
    15. Liu, D., Raghothama, K.G., Hasegawa, P.M., Bressan, R.A.: Osmotin overexpression in potato delays development of disease symptoms.-Proc. nat. Acad. Sci. USA 91: 1888-1892, 1994. Go to original source...
    16. Malamy, J., Carr, J.P., Klessig, D.F., Raskin, I.: Salicylic acid: A likely endogenous signal in the resistance response of tobacco to viral infection.-Science 250: 1002-1004, 1990. Go to original source...
    17. Malamy, J., Klessig, D. F.: Salicylic acid and plant disease resistance.-Plant J. 2: 643-654, 1992. Go to original source...
    18. Mauch-Mani, B., Metraux, J.P.: Salicylic acid and systemic acquired resistance to pathogen attack.-Ann. Bot. 82: 535-540, 1998. Go to original source...
    19. Meena, B., Marimuthu, T., Velazhahan, R.: Salicylic acid induces systemic resistance in groundnut against late leaf spot caused by Cercosporidium personatum.-Indian J.Mycol. Plant Pathol. 31: 139-145, 2001.
    20. Metraux, J.P., Nawrath, C., Genoud, T.: Systemic acquired resistance.-Euphytica 124: 237-243, 2002. Go to original source...
    21. Mills, P.R., Wood, R.K.S.: The effects of polyacrylic acid, aspirin and salicylic acid on resistance of cucumber to Colletotrichum lagenarium.-Phytopathol. Z. 111: 209-216, 1984. Go to original source...
    22. Muthukrishnan, S., Liang, G.H., Trick, H.N., Gill, B.S.: Pathogenesis-related proteins and their genes in cereals.-Plant Cell Tissue Organ Cult. 64: 93-114, 2001. Go to original source...
    23. Nakamura, Y., Sawada, H., Kobayashi, S., Nakajima, I., Yoshikawa, M.: Expression of soybean β-1,3-endoglucanase cDNA and effect on disease tolerance in kiwifruit plants.-Plant Cell Rep. 18: 527-532, 1999. Go to original source...
    24. Nelson, L.R., Marshall, D.: Breeding wheat for resistance to Septoria nodorum and Septoria tritici.-Adv. Agron. 44: 257-277, 1990. Go to original source...
    25. Poole, P.R., McLeod, L.C.: Development of post-harvest resistance to picking wound entry Botrytis cinerea infection in kiwifruit.-New Zeal. J. Crop hort. Sci. 22: 387-392, 1994. Go to original source...
    26. Rakwal, R., Agrawal, G.K., Yonekura, M.: Separation of proteins from stressed rice (Oryza sativa L.) leaf tissues by two-dimensional polyacrylamide gel electrophoresis: induction of pathogenesis-related and cellular protectant proteins by jasmonic acid, UV irradiation and copper chloride.-Electrophoresis 20: 3472-3478, 1999. Go to original source...
    27. Reglinski, T., Poole, P.R., Whitaker, G., Hoyte, S.M.: Induced resistance against Sclerotinia sclerotiorum in kiwifruit leaves.-Plant Pathol. 46: 716-721, 1997. Go to original source...
    28. Roberts, W.K., Selitrennikoff, C.P.: Zeamatin, an antifungal protein from maize with membrane permeabilizing activity.-J. gen. Microbiol. 136: 1771-1778, 1990. Go to original source...
    29. Ryals, J.A., Neuenschwander, U.H., Willits, M.G., Molina, A., Steiner, H., Hunt, M.D.: Systemic acquired resistance.-Plant Cell 8: 1809-1819, 1996. Go to original source...
    30. Saari, E.E., Prescott, J.M.: A scale for appraising the foliar intensity of wheat diseases.-Plant Dis. Rep. 5: 377-380, 1975.
    31. Schweizer, P., Gees, R. E. M., Mosinger, E.: Effect of jasmonic acid on the interaction of barley (Hordeum vulgare L.) with the powdery mildew Erysiphe graminis f. sp. hordei.-Plant Physiol. 102: 503-511, 1993. Go to original source...
    32. Shah, D.A., Bergstrom, G.C., Sorrells, M.E.: Differential seed infection of wheat cultivars by Stagonospora nodorum.-Plant Dis. 84: 749-752, 2000. Go to original source...
    33. Takeuchi, Y., Yoshikawa, M., Takeba, G., Tanaka, K., Shibaba, D., Horino, O.: Molecular cloning and ethylene induction of mRNA encoding a phytoalexin elicitor-releasing factor, β-1,3-endoglucanase, in soybean.-Plant Physiol. 93: 673-682, 1990. Go to original source...
    34. Velazhahan, R., Datta, S. K., Muthukrishnan, S.: The PR-5 family: thaumatin-like proteins.-In: Datta, S.K., Muthukrishnan, S. (ed.): Pathogenesis Related Proteins in Plants. Pp. 107-129. CRC Press, Boca Raton 1999.
    35. Velazhahan, R., Jayaraj, J., Jeoung, J.M., Liang, G.H., Muthukrishnan, S.: Purification and characterization of an antifungal thaumatin-like protein from sorghum (Sorghum bicolor) leaves.-J. Plant Dis. Protect. 109: 452-461, 2002.
    36. Velazhahan, R., Jayaraj, J., Liang, G.H., Muthukrishnan, S.: Partial purification and N-terminal amino acid sequencing of a β-1,3-glucanase from sorghum leaves.-Biol. Plant. 46: 29-33, 2003. Go to original source...
    37. Velazhahan, R., Muthukrishnan, S.: Transgenic tobacco plants constitutively overexpressing a rice thaumatin-like protein (PR-5) show enhanced resistance to Alternaria alternata.-Biol. Plant. 47: 347-354, 2003/4. Go to original source...
    38. Vigers, A.J., Roberts, W.K., Selitrennikoff, C.P.: A new family of plant antifungal proteins.-Mol. Plant-Microbe Interact. 4: 315-323, 1991. Go to original source...
    39. Ward, E., Uknes, S.J., Williams, S.C., Dincher, S.S., Wiederhold, D.L., Alexander, D.C., Ahl-Goy, P., Metraux, J.P., Ryals, J.A.: Coordinate gene activity in response to agents that induce systemic acquired resistance.-Plant Cell 3: 1085-1094, 1991. Go to original source...
    40. Wessels, J.G.H.: Wall growth, protein extraction and morphogenesis in fungi.-New Phytol. 123: 397-413, 1993. Go to original source...
    41. Yalpani, N., Silverman, P., Michael, T., Wilson, A., Kleier, D.A., Raskin, I.: Salicylic acid is a systemic signal and an inducer of pathogenesis-related proteins in virus-infected tobacco.-Plant Cell 3: 809-818, 1991. Go to original source...
    42. Yoshikawa, M., Takeuchi, Y., Horino, O.: A mechanism for ethylene-induced disease resistance in soybean: Enhanced synthesis of an elicitor-releasing factor, β-1,3-endoglucanase.-Physiol. mol. Plant Pathol. 37: 367-376, 1990. Go to original source...

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