biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 48:441-444, 2004 | DOI: 10.1023/B:BIOP.0000041100.94688.2d

In vitro Crown Galls Induced by Agrobacterium tumefaciens Strain A281 (pTiBo542) in Trigonella foenum-graecum

K.M. Khawar1, S. Gulbitti-Onarici2, S. Çöçü1, S. Erisen3, C. Sancak1, S. Özcan1
1 Department of Field Crops, Faculty of Agriculture, University of Ankara, Turkey, e-mail
2 Department of Molecular Biology, Faculty of Science, Hacettepe University, Beytepe Ankara, Turkey
3 Department of Biology, Faculty of Science, Gazi University, Ankara, Turkey

Transformation of fenugreek (Trigonella foenumgraecum) was carried out with A281 oncogenic strain of Agrobacterium tumefaciens using root, cotyledon and hypocotyl explants excised from 1-week-old seedlings, which showed that the plant was highly susceptible to transformation. Tumors (calli) were selected on 50 mg dm-3 kanamycin. They were analyzed for β-glucuronidase (GUS) expression. Presence of uidA (gus) gene, was confirmed by polymerase chain reaction (PCR) amplification.

Keywords: transformation; kanamycin resistance; GUS analysis; PCR
Subjects: Agrobacterium-mediated transformation; Agrobacterium tumefaciens; fenugreek, in vitro crown galls; β-glucuronidase; nutrient medium, Murashige and Skoog; polymerase chain reaction (PCR); transformation, Agrobacterium-mediated; transgenic plants; Trigonella foenum-graecum

Published: September 1, 2004  Show citation

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Khawar, K.M., Gulbitti-Onarici, S., Çöçü, S., Erisen, S., Sancak, C., & Özcan, S. (2004). In vitro Crown Galls Induced by Agrobacterium tumefaciens Strain A281 (pTiBo542) in Trigonella foenum-graecum. Biologia plantarum, 48(3), 441-444. doi: 10.1023/B:BIOP.0000041100.94688.2d
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