biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:473-476, 2004 | DOI: 10.1023/B:BIOP.0000041108.89399.85

Callus Induction and in vitro Regeneration from Barley Mature Embryos

J.M. Zapata1, B. Sabater1, M. Martín1
1 Departamento de Biología Vegetal, Universidad de Alcalá, Madrid, Spain

We have assayed different combinations of nutrient media and growth regulators to induce callus and plant regeneration from explants of root, shoot and leaf, complete seed, and isolated mature embryo of barley (Hordeum vulgare L. cv. Hassan). The best results were obtained with mature embryo in J25-8 medium supplemented with 2.0 mg dm-3 2,4-dichlorophenoxyacetic acid where about 75 % developed friable calli. Some 80 - 85 % of these calli regenerated barley plants in the same J25-8 medium supplemented with 1.0 mg dm-3 indole-3-butyric acid and 0.1 mg dm-3 kinetin.

Keywords: 2,4-D; Hordeum vulgare; IBA; kinetin; NAA; organogenesis
Subjects: auxins; barley, callus induction; cytokinins; Hordeum vulgare; in vitro culture, callus induction; in vitro culture, from mature embryo and seed; in vitro culture, regeneration, organogenesis; nutrient medium, B5 (Gamborg); nutrient medium, J25-8 (Jensen); nutrient medium, Murashige and Skoog; nutrient medium, N6 (Chu); nutrient medium, SH (Schenk and Hildebrandt)

Published: September 1, 2004  Show citation

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Zapata, J.M., Sabater, B., & Martín, M. (2004). Callus Induction and in vitro Regeneration from Barley Mature Embryos. Biologia plantarum, 48(3), 473-476. doi: 10.1023/B:BIOP.0000041108.89399.85
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