biologia plantarum

International journal on Plant Life established by Bohumil Nėmec in 1959

Biologia plantarum 48:491-495, 2004 | DOI: 10.1023/B:BIOP.0000047142.07987.e1

High Frequency Somatic Embryogenesis in Cotton

Y. Aydin1,2, Z. Ipekci1, T. Talas-Oğraš1, A. Zehir2, K. Bajrovic1,3, N. Gozukirmizi1,4
1 Research Institute for Genetic Engineering and Biotechnology, TUBITAK, Gebze-Kocaeli, Turkey
2 Marmara University, Goztepe, Istanbul, Turkey
3 Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia-Herzegovina
4 Faculty of Science, Istanbul University, Vezneciler, Istanbul, Turkey

A highly reproducible system for efficient somatic embryogenesis was developed to regenerate plantlets from cotton (Gossypium hirsutum L.) cultivars (Nazilli M-503 and Nazilli 143). Shoot apices, hypocotyls and nodes of 10-d-old seedlings were used as explants. High frequency (100 %) embryogenic calli was initiated from all tested explants on Murashige and Skoog (1962) (MS) media supplemented with 1 g dm-3 polyvinylpyrrolidone (PVP), 1 mg dm-3 6-benzylaminopurine (BAP), 0.5 mg dm-3 kinetin for Nazilli M-503 and 1 g dm-3 PVP, 2 mg dm-3 BAP, 0.5 mg dm-3 kinetin for Nazilli-143. Globular stage somatic embryos were produced 4 months after transfer to hormone-free MS medium supplemented with 1 g dm-3 PVP. Subsequent subculture of globular embryos every 3 weeks on hormone-free MS medium led to the development of torpedo and cotyledonary stage embryos with the frequency of 75 and 83.2 % from hypocotyl explants of Nazilli M-503 and Nazilli-143, respectively. Afterwards, mature somatic embryos were isolated and cultured on hormone-free MS medium for germination and development into plantlets. The highest germination frequency (42.9 %) for Nazilli M-503 somatic embryos were obtained on hormone-free MS medium after 5 months with hypocotyl explants, whereas germination frequencies of Nazilli-143 embryos from hypocotyl, node and apex explants varied between 22 - 30 %.

Keywords: Gossypium hirsutum L.; indirect somatic embryogenesis; hypocotyls; cotyledonary node; apex
Subjects: cotton, somatic embryology; cultivar and genotype differences, high frequency somatic embryogenesis; cytokinins; Gossypium hirsutum; in vitro culture, somatic embryogenesis; nutrient medium, Murashige and Skoog; somatic embryology

Published: December 1, 2004  Show citation

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Aydin, Y., Ipekci, Z., Talas-Oğraš, T., Zehir, A., Bajrovic, K., & Gozukirmizi, N. (2004). High Frequency Somatic Embryogenesis in Cotton. Biologia plantarum, 48(4), 491-495. doi: 10.1023/B:BIOP.0000047142.07987.e1
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