biologia plantarum

International journal on Plant Life established by Bohumil Nėmec in 1959

Biologia plantarum 48:517-522, 2004 | DOI: 10.1023/B:BIOP.0000047146.69936.ed

Semi-Automated Simple Sequence Repeat Analysis Reveals Narrow Genetic Base in Indian Potato Cultivars

V.P. Chimote1, S.K. Chakrabarti1, D. Pattanayak1, P.S. Naik1
1 Division of Crop Improvement, Central Potato Research Institute, Shimla, India

A study was conducted to generate fingerprints of thirty-two Indian potato cultivars using capillary electrophoresis based semi-automated simple sequence repeat analysis. Five fluorescent-tagged primer pairs (STPOACUTR, LEGAST1, STPOAC58, STM0030 and STM0031) used in this study yielded 43 alleles at 16 different loci, showing multi-loci resolving character. The estimated similarity between the cultivars was in the range of 0.72 to 0.98 indicating narrow genetic relationship. None of the primer set alone could differentiate all 32 cultivars included in this study. However, two primer sets STM-0031 and STPOAC58 amplifying 12 and 9 polymorphic alleles, respectively, could together distinguish all of them. The results indicated usefulness of semi-automated capillary electrophoresis in quick and reproducible SSR genotyping of potato cultivars.

Keywords: genetic relationship; microsatellites; simple sequence repeats polymorphism; Solanum tuberosum
Subjects: amplified fragment length polymorphism (AFLP); cultivar and genotype differences, simple sequence repeats; dendrogram, phylogenetic tree; microsatellites; phylogenetic tree, dendrogram; polymerase chain reaction (PCR); random amplified polymorphic DNA (RAPD) markers; restriction fragment length polymorphism (RFLP); simple sequence repeat; Solanum tuberosum

Published: December 1, 2004  Show citation

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Chimote, V.P., Chakrabarti, S.K., Pattanayak, D., & Naik, P.S. (2004). Semi-Automated Simple Sequence Repeat Analysis Reveals Narrow Genetic Base in Indian Potato Cultivars. Biologia plantarum, 48(4), 517-522. doi: 10.1023/B:BIOP.0000047146.69936.ed
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