biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:523-529, 2004 | DOI: 10.1023/B:BIOP.0000047147.29552.fd

The Role of Cytokinins and Ethylene in Bean Cotyledon Senescence. The Effect of Free Radicals

N. Wilhelmová1, D. Procházková1, I. Macháčková1, M. Vágner1, M. Srbová2, J. Wilhelm2
1 Laboratory of Stress Physiology, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 6, Czech Republic
2 2nd Medical School, Charles University, Praha 5, Czech Republic

During ageing of bean (Phaseolus vulgaris L.) cotyledons in plants with modified life span the time-course of four cytokinins, ethylene, and the end products of free radical attack, lipofuscin-like pigments (LFP), were studied. UV irradiation shortened cotyledon life span, while epicotyl decapitation prolonged it. In controls, LFP increased at the senescence onset but at the end of life span it returned to the initial level. Ethylene increased more than 3-fold at the time of abscission. The content of individual cytokinins (zeatin, zeatin riboside, isopentenyl adenine, isopentenyl adenine riboside) varied differently during ageing but they did not decreased in any case under level observed in young cotyledons at the time of abscission. UV irradiation resulted in 14-fold increase in LFP concentration at the end. Ethylene increased 8-fold 2 h after irradiation. Individual cytokinins increased after UV irradiation to a different extent and time-course, nevertheless cotyledon life span was shortened. Decapitation induced LFP decrease. On day 13, LFP abruptly increased and than decreased and stayed lowered until abscission. Ethylene was maximum on day 24, at the time of abscission, it was above 200 % of control. Decapitation produced transient decrease in some cytokinins namely zeatin and isopentenyl adenine riboside.

Keywords: ageing epicotyl decapitation; lipid peroxidation; lipofuscin-like pigments; Phaseolus vulgaris; UV irradiation
Subjects: abscisic acid (ABA); ageing, epicotyl decapitation; chlorophyll, cotyledon senescence; cotyledons; cytokinins; ethylene, bean cotyledon senescence; free radicals; French bean, ethylene, senescence; life span, cytokinins, chlorophyll; lipid peroxidation; lipofuscin-like pigments; monochromatic radiation; nutrient medium, Hewitt; Phaseolus vulgaris; radiation UV-C, ethylene, senescence; UV irradiation, shortened life span

Published: December 1, 2004  Show citation

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Wilhelmová, N., Procházková, D., Macháčková, I., Vágner, M., Srbová, M., & Wilhelm, J. (2004). The Role of Cytokinins and Ethylene in Bean Cotyledon Senescence. The Effect of Free Radicals. Biologia plantarum, 48(4), 523-529. doi: 10.1023/B:BIOP.0000047147.29552.fd
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