biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 48:569-574, 2004 | DOI: 10.1023/B:BIOP.0000047154.16119.32

Calcium Ameliorates Effects of Lead in Protonema of Funaria hygrometrica Hedw.

M. Krzes³owska1, A. Wo¼ny1, J. Konieczna-Koperska1
1 Institute of Experimental Biology, Adam Mickiewicz University, Collegium Biologicum, Poznañ, Poland

Two-days-old in vitro grown protonemata of Funaria hygrometrica Hedw. were treated with a mixture PbCl2 (4 μM Pb2+) and CaCl2 (16 μM Ca2+) (Ca+Pb) for 48 h. The results were compared with the control: distilled water (H2O) and the solution of PbCl2 (4 μM Pb2+) (Pb). Protonemata treated with Ca+Pb were longer and contained more cells than those treated with Pb. Moreover, a lower number of cells showed apical cell deformations typical for lead toxicity: swollen tips and wall thickenings at the apex. If deformations were present they were not as extended as in Pb. In comparison with the control, however, protonemata treated with Ca+Pb were shorter, contained a lower number of cells and some apical cells in this material were altered. It can be concluded that the presence of calcium partially neutralised toxic effects of lead in Funaria hygrometrica protonemata cells.

Keywords: heavy metals; cell wall; tip growth
Subjects: calcium, amelioration of heavy metals; cell wall; Funaria hygrometrica; growth, tip, heavy metals; heavy metals; lead, protonema, calcium; nutrient medium, Kofler; protonema, lead effect

Published: December 1, 2004  Show citation

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Krzes³owska, M., Wo¼ny, A., & Konieczna-Koperska, J. (2004). Calcium Ameliorates Effects of Lead in Protonema of Funaria hygrometrica Hedw. Biologia plantarum, 48(4), 569-574. doi: 10.1023/B:BIOP.0000047154.16119.32
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