biologia plantarum

International journal on Plant Life established by Bohumil Nėmec in 1959

Biologia plantarum 48:583-588, 2004 | DOI: 10.1023/B:BIOP.0000047155.53756.63

Screening Antimicrobial Activities of Basic Protein Fractions from Dry and Germinated Wheat Seeds

T. Talas-Oğraš1
1 Research Institute for Genetic Engineering and Biotechnology (RIGEB), TUBITAK, Kocaeli, Turkey

Two small cationic peptide fractions (5 kDa) were isolated from dry and germinated seeds of wheat, named WAP and GWAP, respectively. The antifungal and antibacterial activities of the peptides were analyzed using disk diffusion and turbidity measurement assays. The peptides in vitro exhibited effective antifungal activity against four plant pathogenic fungi at minimum concentration of 15 μg(protein) cm-3. Their antimicrobial activity was negatively affected by the presence of 5 mM CaCl2. The peptides were less effective against Gram-negative bacterium Erwinia carotovora subsp. carotovora, but they demonstrated inhibitory activity against Gram-positive bacterium Clavibacter michiganensis subsp. sepedonicus. The antimicrobial activity of GWAP was more effective than WAP.

Keywords: antifungal, antibacterial activity; Triticum durum
Subjects: antifungal and antimicrobial activity, peptides, germination; Botrytis cinerea; Clavibacter michiganensis ssp. sepedonicus; Erwinia carotovora ssp. carotovora; Fusarium oxysporum; germination; Rhizoctonia solani; seed, antibacterial and antifungal activities; Triticum durum; Verticillium dahliae; wheat, seed, antibacterial and antifungal activities

Published: December 1, 2004  Show citation

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Talas-Oğraš, T. (2004). Screening Antimicrobial Activities of Basic Protein Fractions from Dry and Germinated Wheat Seeds. Biologia plantarum, 48(4), 583-588. doi: 10.1023/B:BIOP.0000047155.53756.63
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