biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:589-592, 2004 | DOI: 10.1023/B:BIOP.0000047156.57328.27

Micropropagation of a Local Olive Cultivar for Germplasm Preservation

M. Zacchini1, M. De Agazio1
1 Istituto di Biologia Agroambientale e Forestale, Consiglio Nazionale delle Ricerche, Roma, Italy

In vitro shoot culture was applied to an Italian local cultivar Nebbiara of olive (Olea europaea L.) to preserve its endangered germplasm. This cultivar showed a notable difficulty for the in vitro establishment due to heavy pathogen contamination. Mercury chloride and sodium hypochloride in the sterilisation step and antibiotics in culture media allowed to overcome the problem. Proliferation of shoot apical bud on olive culture medium with 36 g dm-3 mannitol and 4.56 μM zeatin appeared very satisfactory. All the explants tested rooted during a subculture (1 month) preceeded by a 5-d long dark pre-treatment.

Keywords: axillary buds; culture media; in vitro establishment; in vitro rooting; nodal segments
Subjects: in vitro culture, from node and internode; in vitro culture, from shoot-tip, bud; in vitro culture, micropropagation; in vitro culture, root, rooting; in vitro culture, vessel closure-types; nodal segments; nutrient medium, Murashige and Skoog; nutrient medium, olive proliferation medium, OM (Rugini); Olea europea; olive, germplasm preservation; vessel closure types, in vitro culture

Published: December 1, 2004  Show citation

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Zacchini, M., & De Agazio, M. (2004). Micropropagation of a Local Olive Cultivar for Germplasm Preservation. Biologia plantarum, 48(4), 589-592. doi: 10.1023/B:BIOP.0000047156.57328.27
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