biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:621-624, 2004 | DOI: 10.1023/B:BIOP.0000047164.06779.27

In vitro Growth and Shoot Multiplication of Achras zapota in a Controlled Carbon Dioxide Environment

N. Dave1, S.D. Purohit1
1 Plant Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur, India

The culture vessels with multiplying shoots of Achras zapota L. on Schenk and Hildebrandt (SH) medium containing 8.88 μM 6-benzylaminopurine (BAP) with or without sucrose were kept under varied CO2 concentrations ranging from 0.6 to 40.0 g m-3 using different concentrations of sodium bicarbonate (NaHCO3), sodium carbonate (Na2CO3), potassium bicarbonate (KHCO3), and potassium carbonate (K2CO3) in small acrylic chambers. Complete absence of carbon source caused death of shoots within 20 d. Under elevated concentrations of CO2 (10.0 and 40.0 g m-3) the shoots grew photoautotrophically on sucrose-free medium. The growth of cultures was better at 40.0 g (CO2) m-3 than on 3.0 % sucrose under ambient air of growth room. However, the best response was obtained at 10.0 g (CO2) m-3 and 3.0 % sucrose where maximum number of shoots, shoot length, fresh and dry mass, total number of leaves and leaf area was observed.

Keywords: hardening and acclimatization; micropropagation; photoautotrophy; sapota; shoot culture
Subjects: Achras sapota (zapota); cytokinins; hardening and acclimatizetion; in vitro culture, controlled CO2 concentration; in vitro culture, micropropagation; in vitro culture, shoot multiplication; Manilkara zapota; nutrient medium, SH (Schenk and Hildebrandt); nutrient medium, standard multiplication medium, SM (Purohit and Singvi); sapota, in vitro culture

Published: December 1, 2004  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Dave, N., & Purohit, S.D. (2004). In vitro Growth and Shoot Multiplication of Achras zapota in a Controlled Carbon Dioxide Environment. Biologia plantarum, 48(4), 621-624. doi: 10.1023/B:BIOP.0000047164.06779.27
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aitken-Christie, J., Davies, H., Kubota, C., Kozai, T.: Benefits of using a microporous membrane in tissue culture vessel lid for the micropropagation of Pinus radiata.-In: Van der Plas, L.H.W., Aartrijk, J.V., Nijkamp, H.J.J. (ed.): Progress in Plant Cellular and Molecular Biology: Proceedings of the VII International Congress on Plant Tissue and Cell Culture. Pp. 86. Kluwer Academic Publishers, Amsterdam 1990.
    2. Deng, R., Donnelly, D.J.: In vitro hardening of red raspberry through CO2 enrichment and relative humidity reduction on sugar-free medium.-Can. J. Plant Sci. 73: 1105-1113, 1993. Go to original source...
    3. De Proft, M.P., Maene, L.J., Debergh, P.C.: Carbon dioxide and ethylene evolution in culture atmosphere of Magnolia cultured in vitro.-Physiol. Plant. 65: 375-379, 1985. Go to original source...
    4. Doi, M., Oda, H., Asahira, T.: In vitro atmosphere of cultured C3 and CAM plants in relation to day-lengths.-Environ. Control Biol. 27: 9-13, 1989. Go to original source...
    5. Fujiwara, K., Kozai, T., Watanabe, I.: Fundamental studies on environments in plant tissue culture vessels (3). Measurements of carbon dioxide gas concentration in closed vessels containing tissue cultured plantlets and estimates of net photosynthetic rates of the plantlets.-J. Agr. Meteorol. 43: 21-30, 1987. Go to original source...
    6. Fujiwara, K., Kozai, T., Watanabe, I.: Development of a photoautotrophic tissue culture system for shoot and/or plantlets at rooting and acclimatization stages.-Acta Hort. 230: 153-158, 1988. Go to original source...
    7. Jeong, B.R., Fujiwara, K., Kozai, T.: Carbon dioxide enrichment in autotrophic micropropagation: methods and advantages.-Hort. Technol. 3: 332-334, 1993. Go to original source...
    8. Kirdmanee, C., Kitaya, Y., Kozai, T.: Effect of CO2 enrichment and supporting material in vitro on photoautotrophic growth of Eucalyptus plantlets in vitro and ex vitro.-In vitro cell. dev. Biol. Plant 31: 144-149, 1995. Go to original source...
    9. Kozai, T.: Photoautotrophic micropropagation.-In Vitro cell. dev. Biol. Plant 27: 47-51, 1991. Go to original source...
    10. Kozai, T., Fujiwara, K., Hayashi, M., Aitken-Christie, J.: The in vitro environment and its control in micropropagation.-In: Kurata, K., Kozai, T. (ed.): Transplant Production System. Pp. 247-282. Kluwer Academic Publishers, Dordrecht 1992. Go to original source...
    11. Kozai, T., Iwanami, Y.: Effects of CO2 enrichment and sucrose concentration under high photon fluxes on plantlet growth of carnation (Dianthus caryophyllus L.) in tissue culture during preparation stage.-J. Jap. Soc. Hort. Sci. 57: 279-288, 1988. Go to original source...
    12. Langford, P.J., Wainwright, H.: Effects of sucrose concentration on the photosynthetic ability of rose shoots in vitro.-Ann. Bot. 60: 633-640, 1987. Go to original source...
    13. Morini, S., Melai, M.: CO2 dynamics and growth in photoautotrophic and photomixotrophic apple cultures.-Biol. Plant. 47: 167-172, 2003/4. Go to original source...
    14. Mousseau, M.: CO2 enrichment in vitro: effect on autotrophic and heterotrophic cultures of Nicotiana tabacum.-Photosynth. Res. 8: 187-191, 1986. Go to original source...
    15. Purohit, S.D., Singhvi, A.: Micropropagation of Achras sapota through enhanced axillary branching.-Scientia Hort. 76: 219-229, 1998. Go to original source...
    16. Sha Valli Khan, P.S., Kozai, T., Nguyen, Q.T., Kubota, C., Dhawan, V.: Growth and water relations of Paulownia fortunei under photomixotrophic and photoautotrophic conditions.-Biol. Plant. 46: 161-166, 2003. Go to original source...
    17. Schenk, R.V., Hildebrandt, A.C.: Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures.-Can. J. Bot. 50: 199-204, 1972. Go to original source...
    18. Solárová, J., Pospíšilová, J.: Effect of carbon dioxide enrichment during in vitro cultivation and acclimation to ex vitro conditions.-Biol. Plant. 39: 23-30, 1997. Go to original source...
    19. Solárová, J., Pospíšilová, J., Čatský, J. SantrŮček, J.: Photosynthesis and growth of tobacco plantlets in dependence on carbon supply.-Photosynthetica 23: 629-637, 1989.
    20. Solárová, J., Součková, D., Ullmann, J., Pospíšilová, J.: In vitro culture: environmental conditions and plantlet growth as affected by vessel type and stopper material.-Zahradnictví (Praha) 23: 51-58, 1996.
    21. Thompson, M.R., Thorpe, T.A.: Metabolic and non-metabolic roles of carbohydrates.-In: Bonga, J.M., Durzan, D.J. (ed.): Cell and Tissue Culture in Forestry. Pp. 89-112. Martinus Nijhoff, Dordrecht 1987. Go to original source...
    22. Tichá, I.: Optimization of photoautotrophic tobacco in vitro culture: effect of suncaps closures on plantlet growth.-Photosynthetica 32: 475-479, 1996.
    23. Vasil, I.: Cell Culture and Somatic Cell Genetics of Plants. Vol. 8.-Academic Press, New York 1991.
    24. Vyas, S., Purohit, S.D.: In vitro growth and shoot multiplication of Wrightia tomentosa Roem et Schult in a controlled carbon dioxide environment.-Plant Cell Tissue Organ Cult. 75: 283-286, 2003. Go to original source...
    25. Woltering, E.J.: Ethylene and carbon dioxide accumulation within various tissue culture systems.-Acta. bot. neerl. 35: 50, 1986.

    Return to the content