biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:633-636, 2004 | DOI: 10.1023/B:BIOP.0000047167.18592.f7

Micropropagation of Endangered Species Daphne cneorum

J. Malá1, V. Bylinský2
1 Forestry and Game Management Research Institute, Jíloviště - Strnady
2 Agency for Protection of Nature and Countryside, Czech Republic

A new protocol for micropropagation of endangered Daphne cneorum through multiple shoot formation has been developed. Two different types of explants (dormant apical buds and in vitro seed-derived young seedlings) from plants in two different localities were used for the initiation of multiple shoots on agar woody plant medium (WPM) with 0.2 mg dm-3 benzylaminopurine (BAP), 0.1 mg dm-3β-indolebutyric acid (IBA), 200 mg dm-3 glutamine, and 200 mg dm-3 casein hydrolysate. From 10 seeds only one germinated and the multi-apex culture bearing 12 shoots sprouted out from in vitro seed-derived young seedling. After 6-month cultivation 35 multi-apex cultures were achieved from in vitro seed-derived young seedling. On 1/3 strength WPM medium supplemented with 2.83 mg dm-3 IBA 50 % of cultures (clusters of 3 - 5 shoots) rooted but no rooting occurred in the presence of α-naphthaleneacetic acid (NAA). The rooted plantlets were acclimatized for 4 weeks in the greenhouse and then transferred into natural conditions. The plants successfully survived the winter and flowered.

Keywords: plantlet regeneration; organogenesis; growth regulators

Published: December 1, 2004  Show citation

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Malá, J., & Bylinský, V. (2004). Micropropagation of Endangered Species Daphne cneorum. Biologia plantarum, 48(4), 633-636. doi: 10.1023/B:BIOP.0000047167.18592.f7
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