biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 49:1-6, 2005 | DOI: 10.1007/s10535-005-1006-5

Somatic embryogenesis from ovaries, developing ovules and immature zygotic embryos, and improved embryo development of Castanea sativa

U. Sauer1, E. Wilhelm1,*
1 Austrian Research Centre Seibersdorf, Seibersdorf, Austria

Somatic embryogenesis of European chestnut (Castanea sativa Mill.) was obtained using juvenile tissue cultured on P24 medium with 5 μM 2,4-dichlorophenoxyacetic acid plus 0.5 μM 6-benzylaminopurine (BA) for three weeks and then cultured on 0.89 μM BA. Induction frequency with ovaries ranged from 2.0 to 19.1 % and was observed in tissue collected 2 to 8 weeks postanthesis, ovules used as a starting tissue gained 0.8 to 7.8 %, 3 to 9 weeks postanthesis. Zygotic embryos collected 5 to 10 weeks postanthesis formed 10.5 to 57.1 % somatic embryos, respectively. The culture lines were maintained via secondary embryogenesis on P24 medium with 0.89 μM BA. Development and maturation were stimulated on P24 medium with increased agar concentration (1.1 %). Five plantlets were transferred to substrate and acclimatized successfully in greenhouse.

Keywords: 6-benzylaminopurine; 2; 4-dichlorophenoxyacetic acid; European chestnut; Fagaceae
Subjects: Castanea sativa; cytokinins; embryo development, somatic; European chestnut; germination; gibberellic acid, gibberellins; in vitro culture, from immature embryos; in vitro culture, from ovaries, ovules; in vitro culture, somatic embryogenesis; nutrient medium GD (Gresshof and Doy); nutrient medium WPM (woody plant medium); zygotic embryos

Received: July 23, 2003; Accepted: June 9, 2004; Published: March 1, 2005  Show citation

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Sauer, U., & Wilhelm, E. (2005). Somatic embryogenesis from ovaries, developing ovules and immature zygotic embryos, and improved embryo development of Castanea sativa. Biologia plantarum, 49(1), 1-6. doi: 10.1007/s10535-005-1006-5
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